Light fractionated ALA-PDT enhances therapeutic efficacy in vitro; the influence of PpIX concentration and illumination parameters

被引：25
作者：
de Bruijn, Henriette S. [1 ]
Casas, Adriana G. [2 ]
Di Venosa, Gabriela [2 ]
Gandara, Lautato [2 ]
Sterenborg, Henricus J. C. M. [1 ]
Batlle, Alcira [2 ]
Robinson, Dominic J. [1 ]
机构：
[1] Erasmus MC, Ctr Opt Diagnost & Therapy, Dept Radiat Oncol, NL-3000 CA Rotterdam, Netherlands
[2] CONICET Hosp Clin Gral Jose de San Martin, Ctr Invest Porfirinas & Porfirias CIPYP, Buenos Aires, DF, Argentina
来源：
PHOTOCHEMICAL & PHOTOBIOLOGICAL SCIENCES | 2013年 / 12卷 / 02期
关键词：
MEDIATED PHOTODYNAMIC THERAPY; TOPICAL 5-AMINOLEVULINIC ACID; BASAL-CELL CARCINOMA; HAIRLESS MOUSE SKIN; AMINOLEVULINIC-ACID; PROTOPORPHYRIN-IX; SUBCELLULAR-LOCALIZATION; FLUORESCENCE KINETICS; SINGLET OXYGEN; MITOCHONDRIAL;
D O I：
10.1039/c2pp25287b
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Light fractionation, with a long dark interval, significantly increases the response to ALA-PDT in pre-clinical models and in non-melanoma skin cancer. We investigated if this increase in efficacy can be replicated in PAM 212 cells in vitro. The results show a significant decrease in cell survival after light fractionation which is dependent on the PpIX concentration and light dose of the first light fraction. This study supports the hypothesis that an underlying cellular mechanism is involved in the response to light fractionation in which a first light fraction leads to sub-lethally damaged cells that are sensitised to a second light fraction 2 hours later. The current study reveals the in vitro circumstances under which we can investigate the cellular pathways involved.
引用
收藏
页码：241 / 245
页数：5
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