Matrix Metalloproteinases (MMPs) Activity in Cultured Canine Bone Marrow Stromal Cells (BMSCs)

被引：3

作者：

Haraguchi, Tomoya ^{[1
]}

Tani, Kenji ^{[1
]}

Koga, Masato ^{[1
]}

Oda, Yasutaka ^{[1
]}

Itamoto, Kazuhito ^{[2
]}

Yamamoto, Naoki ^{[3
]}

Terai, Shuji ^{[3
]}

Sakaida, Isao ^{[3
]}

Nakazawa, Hiroshi ^{[1
]}

Taura, Yasuho ^{[1
]}

机构：

[1] Yamaguchi Univ, Dept Vet Surg, Fac Agr, Yamaguchi 7538515, Japan

[2] Yamaguchi Univ, Fac Agr, Dept Anim Med Ctr, Yamaguchi 7538515, Japan

[3] Yamaguchi Univ, Grad Sch Med, Dept Gastroenterol & Hepatol, Ube, Yamaguchi 7558505, Japan

来源：

JOURNAL OF VETERINARY MEDICAL SCIENCE | 2012年 / 74卷 / 05期

关键词：

bone marrow stromal cell (BMSC); canine; culture; inflammatory cytokines; matrix metalloproteinase (MMPs); SMOOTH-MUSCLE-CELLS; LIVER-REGENERATION; INTERLEUKIN-6; PROTECTS; STEM-CELLS; TNF-ALPHA; MATRIX-METALLOPROTEINASE-9; FIBROSIS; MICE; HEPATOCYTES; EXPRESSION;

D O I：

10.1292/jvms.11-0395

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

Autologous bone marrow stromal cells (BMSCs) infusion therapy improves the hepatic fibrosis. To investigate the mechanism of remission, we evaluated the matrix metalloproteinase (MMP)-2 and -9 activity in canine BMSCs and the effect of pro-inflammatory cytokines on their expression. The activity and the gene expression of MMPs were analyzed by gelatin zymography and quantitative RT-PCR, respectively. The specific gelatinase bands were indicative effect of MMP-2 and -9 in canine BMSCs. MMP-2 expression seemed to be increased by TNF-alpha and IL-1 beta while MMP-9 was enhanced by TNF-alpha and IL-6. These results suggested that remissive effect on liver fibrosis might be partly attributable to the MMP-2 and -9 activity in BMSCs under the inflammatory condition.

引用

页码：633 / 636

页数：4

共 24 条

[1] Cell differentiation - Hepatocytes from nonhepatic adult stem cells
Alison, MR
Poulsom, R
Jeffery, R
Dhillon, AP
Quaglia, A
Jacob, J
Novelli, M
Prentice, G
Williamson, J
Wright, NA
[J]. NATURE, 2000, 406 (6793) : 257 - 257
[2] Matrix metalloproteinase-9 in lung remodeling
Atkinson, JJ
Senior, RM
[J]. AMERICAN JOURNAL OF RESPIRATORY CELL AND MOLECULAR BIOLOGY, 2003, 28 (01) : 12 - 24
[3] Interleukin-6 protects hepatocytes from CCl4-mediated necrosis and apoptosis in mice by reducing MMP-2 expression
Bansal, MB
Kovalovich, K
Gupta, R
Li, W
Agarwal, A
Radbill, B
Alvarez, CE
Safadi, R
Fiel, MI
Friedman, SL
Taub, RA
[J]. JOURNAL OF HEPATOLOGY, 2005, 42 (04) : 548 - 556
[4] Divergent regulation by growth factors and cytokines of 95 kDa and 72 kDa gelatinases and tissue inhibitors of metalloproteinases-1, -2 and -3 in rabbit aortic smooth muscle cells
Fabunmi, RP
Baker, AH
Murray, EJ
Booth, RFG
Newby, AC
[J]. BIOCHEMICAL JOURNAL, 1996, 315 : 335 - 342
[5] Bone marrow-derived cells express matrix metalloproteinases and contribute to regression of liver fibrosis in mice
Higashiyama, Reiichi
Inagaki, Yutaka
Hong, Yun Yu
Kushida, Miwa
Nakao, Sachie
Niioka, Maki
Watanabe, Tetsu
Okano, Hideyuki
Matsuzaki, Yumi
Shiota, Goshi
Okazaki, Isao
[J]. HEPATOLOGY, 2007, 45 (01) : 213 - 222
[6] Interleukin-6 protects against Fas-mediated death by establishing a critical level of anti-apoptotic hepatic proteins FLIP, Bcl-2, and Bcl-xL
Kovalovich, K
Li, W
DeAngelis, R
Greenbaum, LE
Ciliberto, G
Taub, R
[J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 2001, 276 (28) : 26605 - 26613
[7] Multi-organ, multi-lineage engraftment by a single bone marrow-derived stem cell
Krause, DS
Theise, ND
Collector, MI
Henegariu, O
Hwang, S
Gardner, R
Neutzel, S
Sharkis, SJ
[J]. CELL, 2001, 105 (03) : 369 - 377
[8] PLASMA-LEVELS OF HGF IN RATS TREATED WITH TUMOR PROMOTERS
LINDROOS, P
TSAI, WH
ZARNEGAR, R
MICHALOPOULOS, GK
[J]. CARCINOGENESIS, 1992, 13 (01) : 139 - 141
[9] THE MATRIX-DEGRADING METALLOPROTEINASES
MATRISIAN, LM
[J]. BIOESSAYS, 1992, 14 (07) : 455 - 463
[10] Role of matrix metalloproteinases in normal and disease processes
McDonnell, S
Morgan, M
Lynch, C
[J]. BIOCHEMICAL SOCIETY TRANSACTIONS, 1999, 27 (04) : 734 - 740

← 1 2 3 →