Separation of basic drug enantiomers by capillary zone electrophoresis using ovoglycoprotein as a chiral selector

The utilization of crude ovomucoid from chicken egg whites (OMCHI) for the separation of drug enantiomers as a chiral selector in capillary zone electrophoresis (CZE) proved to be unsatisfactory. Because only about 10% ovoglycoprotein (OGCHI), which is responsible for chiral recognition ability, was included in crude OMCHI, we investigated the utilization of OGCHI as a chiral selector in CZE. To avoid protein adsorption on the wall, two kinds of modified capillaries, a vinyl capillary and a linear polyacrylamide-coated capillary, were employed. Separations of basic drug enantiomers were achieved by optimizing the concentrations of OGCHI and running buffer, running buffer pH, and the nature and content of organic modifier. The present method was successfully applied to the assay of eperisone enantiomers. (C) 1997 Elsevier Science B.V.