A Triggered DNA Hydrogel Cover to Envelop and Release Single Cells

被引：137
作者：
Jin, Juan [1 ,2 ,3 ]
Xing, Yongzheng [1 ]
Xi, Yanli [4 ]
Liu, Xueli [4 ]
Zhou, Tao [1 ]
Ma, Xinxin [1 ]
Yang, Zhongqiang [1 ]
Wang, Shutao [4 ]
Liu, Dongsheng [1 ]
机构：
[1] Tsinghua Univ, Dept Chem, Minist Educ, Key Lab Organ Optoelect & Mol Engn, Beijing 100084, Peoples R China
[2] Natl Ctr Nanosci & Technol, Beijing 100190, Peoples R China
[3] Univ Chinese Acad Sci, Beijing 100049, Peoples R China
[4] Chinese Acad Sci, BNLMS, Key Lab Organ Solids, Inst Chem, Beijing 100190, Peoples R China
来源：
ADVANCED MATERIALS | 2013年 / 25卷 / 34期
基金：
中国国家自然科学基金;
关键词：
DNA hydrogel; envelop and release; permeable; responsiveness; single cell; CROSS-LINKED HYDROGEL; EFFICIENT CAPTURE; APTAMER; ARRAYS;
D O I：
10.1002/adma.201301175
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
We develop an enzyme-triggered permeable DNA hydrogel cover to envelop and release single cells in microwells. The porous structure of the DNA hydrogel allows nutrients and waste to pass through, leading to a cell viability as high as 98%. The design provides a general method to culture, monitor, and manipulate single cells, and has potential applications in cell patterning and studying cell communication. Copyright © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
引用
收藏
页码：4714 / 4717
页数：4
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