Molecular identification and antibiotic resistance of bacteriocinogenic lactic acid bacteria isolated from table olives

In the present study, lactic acid bacteria were isolated from table olive in Morocco. Random Amplified Polymorphic DNA fingerprinting with (GTG)'(5) primer revealed a remarquable variability within isolates. According to the molecular identification,Enterococcus faeciumwas the most dominant species isolated with 32 strains (84.21%), followed by 4 strains ofWeissella paramesenteroides(10.52%), 1 strain ofLeuconostoc mesenteroides(2.63%) andLactobacillus plantarum(2.63%). All of the strains that were identified showed occurrence of more than one bacteriocin-encoding gene. Based on the results obtained,L. plantarum11 showed a mosaic of loci coding for nine bacteriocins (pln A, pln D,pln K, pln G, pln B, pln C, pln N, pln J, ent P). A phenotypic and genotypic antibiotic resistance was also examined.L. plantarum11,L. mesenteroides62,W. paramesenteroides9 andW. paramesenteroides36 as well as all the strains ofE. faeciumwere susceptible to ampicillin, clindamycin and teicoplanin; however, isolates showed a resistance profile against tetracycline and erythromycin. ExceptE. faecium114,E. faecium130 andL. plantarum11, no antibiotic resistance genes were detected in all of the strains, which might be due to resistances resulting from non-transferable or non-acquired resistance determinants (intrinsic mechanism).