15,16-Dihydrotanshinone I-induced Apoptosis in Human Colorectal Cancer Cells: Involvement of ATF3

被引：0

作者：

Suk, Fat-Moon ^{[1
]}

Jou, Wen-Ju ^{[2
]}

Lin, Ren-Jye ^{[3
]}

Lin, Shyr-Yi ^{[3
]}

Tzeng, Fang-Yu ^{[5
]}

Liang, Yu-Chih ^{[4
,5
]}

机构：

[1] Taipei Med Univ, Wan Fang Hosp, Dept Internal Med, Div Gastroenterol, Taipei 11031, Taiwan

[2] New Taipei City Hosp, Dept Internal Med, Div Pulm, New Taipei City, Taiwan

[3] Taipei Med Univ Hosp, Dept Primary Care Med, Taipei, Taiwan

[4] Taipei Med Univ Hosp, Tradit Herbal Med Res Ctr, Taipei, Taiwan

[5] Taipei Med Univ, Coll Med Sci & Technol, Sch Med Lab Sci & Biotechnol, Taipei 11031, Taiwan

来源：

ANTICANCER RESEARCH | 2013年 / 33卷 / 08期

关键词：

15,16-Dihydrotanshinone I; colorectal cancer; activating transcription factor-3; SW480; SW620; SW420; cells; ACTIVATING TRANSCRIPTION FACTOR-3; DIFFERENTIAL REGULATION; GENE; EXPRESSION; IDENTIFICATION; SUPPRESSES; BIOLOGY; PROTEIN;

D O I：

暂无

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

15,16-Dihydrotanshinone I (DHTS) is a component of the traditional Chinese medicinal plant Salvia miltiorrhiza Bunge. In this study, DHTS at as low as 2.5 mu g/ml concentration significantly inhibited proliferation of human benign (SW480) and malignant (SW620) colorectal cancer cells, as shown by 3-(4,5)-dimethylthiahiazo (-z-yl)-3,5-diphenytetrazoliumromide (MTT) and flow cytometric analysis. Activating transcription factor (ATF)-3, a basic leucine zipper-type transcription factor, was found to be predominantly up-regulated in DHTS-treated SW480 and SW620 cells. The up-regulation of ATF3 was blocked by a c-JUN N-terminal kinase (JNK) or p38 inhibitor. Overexpression of ATF3 resulted in a significant augmentation of DHTS-induced apoptosis of SW480 cells, but resistance to DHTS-induced apoptosis of SW620 cells. These results suggest that DHTS has a strong therapeutic or preventive potential against cancer. In addition, ATF3 has a dual role in DHTS-induced apoptosis, depending on the degree of malignancy of colorectal cancer.

引用

页码：3225 / 3231

页数：7

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