Profiling and bioinformatics analyses reveal differential circular RNA expression in NK/T-cell lymphoma-associated hemophagocytic syndrome

被引:0
作者
Man, Changfeng [1 ]
Fan, Yu [2 ]
Yin, Guangli [1 ]
Huang, Jiayu [1 ]
Wang, Jujuan [3 ]
Qiu, Hongxia [1 ,3 ]
机构
[1] Nanjing Med Univ, Jiangsu Prov Hosp, Affiliated Hosp 1, Dept Hematol, 300 Guangzhou Rd, Nanjing 210029, Jiangsu, Peoples R China
[2] Nanjing Med Univ, Jiangsu Univ, Affiliated Peoples Hosp, Dept Canc Inst,Zhenjiang Clin Med Coll, 8 Dianli Rd, Zhenjiang 212002, Jiangsu, Peoples R China
[3] Nanjing Med Univ, Jiangsu Prov Hosp, Affiliated Hosp 1, Dept Geriatr Hematol, 300 Guangzhou Rd, Nanjing 210029, Jiangsu, Peoples R China
关键词
BIOMARKER; CANCER; LYMPHOHISTIOCYTOSIS; PROLIFERATION; LANDSCAPE; DIAGNOSIS; PATHWAY; TARGETS; STAR;
D O I
10.1042/BSR20201590
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Circular RNAs (circRNAs) may be potential biomarkers or therapeutic targets of hemophagocytic syndrome (HPS) due to their high stability, covalently closed structure and implicated roles in gene regulation. The aim of the present study was to determine and characterize the circRNAs from natural killer (NK)/T-cell lymphoma-associated hemophagocytic syndrome (NK/T-LAHS). CircRNA in NK/T-LAHS and healthy control patient serum were assessed using next-generation sequencing (NGS). One hundred and forty-three differentially expressed circRNAs of which 114 were up-regulated and 29 were down-regulated in NK/T-LAHS patients were identified. Next, Gene Ontology (GO) function and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses to explore the roles of these circRNAs were utilized, and a microRNA (miRNA) target gene prediction software to predict the interaction of circRNAs and miRNAs was used. Moreover, five circRNAs were then selected as NK/T-LAHS candidate circRNAs which were related to tumors and contained NK/T-LAHS-related miRNA-binding sites. Using real-time PCR, the significant up-regulation of these five circRNAs in NK/T-LAHS patient serum were verified. Together these results show that circRNAs may serve as valuable diagnostic biomarkers of early NK/T-LAHS, with potential therapeutic targets in disease progression.
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页数:13
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