Development of eighteen polymorphic microsatellite loci for the banded coral shrimp, Stenopus hispidus

被引：1
作者：
Wainwright, Benjamin J. [1 ]
Arlyza, Irma S. [2 ]
Karl, Stephen A. [1 ]
机构：
[1] Univ Hawaii Manoa, Hawaii Inst Marine Biol, Kaneohe, HI 96744 USA
[2] Indonesian Inst Sci, Res Ctr Oceanog, Jakarta 14430, Indonesia
来源：
CONSERVATION GENETICS RESOURCES | 2012年 / 4卷 / 04期
基金：
美国国家科学基金会;
关键词：
Connectivity; Gene flow; Indonesia; Conservation; PRIMERS; DNA;
D O I：
10.1007/s12686-012-9680-z
中图分类号：
X176 [生物多样性保护];
学科分类号：
090705 ;
摘要：
Eighteen polymorphic microsatellite loci were developed for the banded coral shrimp, Stenopus hispidus, using 454 sequencing of microsatellite enriched genomic libraries. The 18 loci were characterized in 24 individuals from the Indonesian Archipelago. We found a mean of 8.7 alleles per locus and observed levels of heterozygosity ranged from 0.21 to 1.00. Two loci deviated significantly from Hardy-Weinberg equilibrium and no significant evidence of linkage disequilibrium was observed.
引用
收藏
页码：947 / 949
页数：3
相关论文
共 13 条
  • [1] [Anonymous], 2010, Geneious v5.5
  • [2] M13-tailed primers improve the readability and usability of microsatellite analyses performed with two different allele-sizing methods
    Boutin-Ganache, I
    Raposo, M
    Raymond, M
    Deschepper, CF
    [J]. BIOTECHNIQUES, 2001, 31 (01) : 24 - +
  • [3] Arlequin suite ver 3.5: a new series of programs to perform population genetics analyses under Linux and Windows
    Excoffier, Laurent
    Lischer, Heidi E. L.
    [J]. MOLECULAR ECOLOGY RESOURCES, 2010, 10 (03) : 564 - 567
  • [4] Fletcher D. J., 1995, International Zoo Yearbook, V34, P73, DOI 10.1111/j.1748-1090.1995.tb00661.x
  • [5] Isolating microsatellite DNA loci
    Glenn, TC
    Schable, NA
    [J]. MOLECULAR EVOLUTION: PRODUCING THE BIOCHEMICAL DATA, PART B, 2005, 395 : 202 - 222
  • [6] Hair C, 2004, WORLDFISH CTR Q, V27
  • [7] Climate change, human impacts, and the resilience of coral reefs
    Hughes, TP
    Baird, AH
    Bellwood, DR
    Card, M
    Connolly, SR
    Folke, C
    Grosberg, R
    Hoegh-Guldberg, O
    Jackson, JBC
    Kleypas, J
    Lough, JM
    Marshall, P
    Nyström, M
    Palumbi, SR
    Pandolfi, JM
    Rosen, B
    Roughgarden, J
    [J]. SCIENCE, 2003, 301 (5635) : 929 - 933
  • [8] Population Connectivity and Conservation of Marine Biodiversity
    Jones, Geoffrey P.
    Srinivasan, Maya
    Almany, Glenn R.
    [J]. OCEANOGRAPHY, 2007, 20 (03) : 100 - 111
  • [9] QDD: a user-friendly program to select microsatellite markers and design primers from large sequencing projects
    Meglecz, Emese
    Costedoat, Caroline
    Dubut, Vincent
    Gilles, Andre
    Malausa, Thibaut
    Pech, Nicolas
    Martin, Jean-Francois
    [J]. BIOINFORMATICS, 2010, 26 (03) : 403 - 404
  • [10] Rozen S, 2000, Methods Mol Biol, V132, P365
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