Nonmuscle myosin heavy chain IIA mediates Epstein-Barr virus infection of nasopharyngeal epithelial cells

被引：78

作者：

Xiong, Dan ^{[1
]}

Du, Yong ^{[1
]}

Wang, Hong-Bo ^{[1
]}

Zhao, Bo ^{[2
,3
]}

Zhang, Hua ^{[1
]}

Li, Yan ^{[1
]}

Hu, Li-Juan ^{[1
]}

Cao, Jing-Yan ^{[1
]}

Zhong, Qian ^{[1
]}

Liu, Wan-Li ^{[1
]}

Li, Man-Zhi ^{[1
]}

Zhu, Xiao-Feng ^{[1
]}

Tsao, Sai Wah ^{[4
,5
]}

Hutt-Fletcher, Lindsey M. ^{[6
]}

Song, Erwei ^{[7
]}

Zeng, Yi-Xin ^{[1
]}

Kieff, Elliott ^{[2
,3
]}

Zeng, Mu-Sheng ^{[1
]}

机构：

[1] Sun Yat Sen Univ, Ctr Canc, Dept Expt Res, State Key Lab Oncol South China,Collaborat Innova, Guangzhou 510060, Guangdong, Peoples R China

[2] Brigham & Womens Hosp, Dept Med & Microbiol & Immunobiol, Boston, MA 02115 USA

[3] Harvard Univ, Sch Med, Boston, MA 02115 USA

[4] Univ Hong Kong, Dept Anat, Hong Kong, Hong Kong, Peoples R China

[5] Univ Hong Kong, Ctr Canc Res, Hong Kong, Hong Kong, Peoples R China

[6] Louisiana State Univ, Dept Microbiol & Immunol, Hlth Sci Ctr, Shreveport, LA 71130 USA

[7] Sun Yat Sen Univ, Sun Yat Sen Mem Hosp, Guangdong Prov Key Lab Malignant Tumor Epigenet &, Guangzhou 510120, Peoples R China

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 2015年 / 112卷 / 35期

基金：

中国国家自然科学基金;

关键词：

Epstein-Barr virus; nasopharyngeal carcinoma; NMHC-IIA; gH/gL; BMI1; B-CELLS; CONFORMATIONAL-CHANGE; GLYCOPROTEINS GHGL; FUSION; ENTRY; EBV; RECEPTOR; CARCINOMA; BINDING; ALPHA-V-BETA-8;

D O I：

10.1073/pnas.1513359112

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

EBV causes B lymphomas and undifferentiated nasopharyngeal carcinoma (NPC). Although the mechanisms by which EBV infects B lymphocytes have been extensively studied, investigation of the mechanisms by which EBV infects nasopharyngeal epithelial cells (NPECs) has only recently been enabled by the successful growth of B lymphoma Mo-MLV insertion region 1 homolog (BMI1)-immortalized NPECs in vitro and the discovery that neuropilin 1 expression positively affects EBV glycoprotein B (gB)-mediated infection and tyrosine kinase activations in enhancing EBV infection of BMI1-immortalized NPECs. We have now found that even though EBV infected NPECs grown as a monolayer at extremely low efficiency (<3%), close to 30% of NPECs grown as sphere-like cells (SLCs) were infected by EBV. We also identified nonmuscle myosin heavy chain IIA (NMHC-IIA) as another NPEC protein important for efficient EBV infection. EBV gH/gL specifically interacted with NMHC-IIA both in vitro and in vivo. NMHC-IIA densely aggregated on the surface of NPEC SLCs and colocalized with EBV. EBV infection of NPEC SLCs was significantly reduced by NMHC-IIA siRNA knock-down. NMHC-IIA antisera also efficiently blocked EBV infection. These data indicate that NMHC-IIA is an important factor for EBV NPEC infection.

引用

页码：11036 / 11041

页数：6

共 40 条

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