A continuous method for measuring calpain activity

被引：20

作者：

Jiang, ST ^{[1
]}

Wang, JH ^{[1
]}

Chang, T ^{[1
]}

Chen, CS ^{[1
]}

机构：

[1] ACAD SINICA,INST BOT,TAIPEI 11529,TAIWAN

来源：

ANALYTICAL BIOCHEMISTRY | 1997年 / 244卷 / 02期

关键词：

D O I：

10.1006/abio.1996.9872

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A simplified methodology for assaying the caseinolysis by calpains was developed. This methodology, including the incubation of calpain with casein and direct measurement of the absorbance at 500 nm, is based on the turbidity of the reaction mixture caused by the aggregation of hydrolysates during the reaction. Unlike the typical caseinolysis assay, this novel assay does not need to separate the substrate from hydrolysates and can be continuously monitored in visible wavelength range. The activity of calpain is expressed by the maximum reaction velocity (Delta A(500)/min) at 25 degrees C. (C) 1997 Academic Press

引用

页码：233 / 238

页数：6

共 32 条

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