The objective of the paper is an attempt at the identification and assessment of factors stimulating and limiting the development of technological entrepreneurship and partnering in the sector of small and medium-sized enterprises in Poland. Meeting this objective required evaluation of factors and methods of technological entrepreneurship development among small and medium-sized companies in Poland (such as an open organizational culture), identification of barriers to the development of an open innovation organizational culture among small and medium-sized enterprises (such as inter-organizational partnering), along with formulation of practical recommendations for the development of technological entrepreneurship, an open organizational culture and partnering in specific enterprises (SMEs), both in terms of cooperation with the R&D, financial and local environment institutions, as well as the development of internal resources and competences to ensure the adaptation of enterprises to the requirements of modern technologies. The study was conducted in 2012-2013 on a sample of 300 small and medium-sized companies located in Poland.