A new quantitative method for rabies virus by detection of nucleoprotein in virion using ELISA

被引：14

作者：

Katayama, S ^{[1
]}

Yamanaka, M ^{[1
]}

Ota, S ^{[1
]}

Shimizu, Y ^{[1
]}

机构：

[1] Kyoto Biken, Div Vet Microbiol, Uji, Kyoto 6110041, Japan

来源：

JOURNAL OF VETERINARY MEDICAL SCIENCE | 1999年 / 61卷 / 04期

关键词：

ELISA; G protein; N protein; rabies virus;

D O I：

10.1292/jvms.61.411

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

We have developed a new quantitative method for rabies virus (RV) detection using enzyme-linked immunosorbent assay (ELISA). The method named N-ELISA was based on the quantitation of nucleoprotein (N) in RV virions captured by RV-specific polyclonal antibodies on an ELISA plate. Both infective and defective interfering (DI) particles of RV could be detected by this method. When viruses were propagated in a medium of pH 7.4 adjusted with 7% NaHCO3, N-ELISA could detect them with titers of more than 10(6) pfu/ml, though the result did not correlate highly with that of the infectivity assay. The reason for this was considered to be that RVs included spikeless and damaged particles which were produced under conditions of low or high pH. However, in the time course of virus yield, titers of N-ELISA correlated well with those of the infectivity assay.

引用

页码：411 / 416

页数：6

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