Study on the interaction between Besifloxacin and bovine serum albumin by spectroscopic techniques

被引:19
作者
Yu, Xianyong [1 ,2 ]
Jiang, Bingfei [1 ]
Liao, Zhixi [1 ]
Jiao, Yue [3 ]
Yi, Pinggui [1 ]
机构
[1] Hunan Univ Sci & Technol, Key Lab Theoret Organ Chem & Funct Mol, Hunan Prov Coll, Minist Educ,Sch Chem & Chem Engn,Key Lab QSAR QSP, Xiangtan 411201, Peoples R China
[2] Fudan Univ, Minist Educ, Key Lab Computat Phys Sci, Shanghai 200433, Peoples R China
[3] Nanjing Chemipioneer Pharma & Tech Co Ltd, Nanjing 210032, Jiangsu, Peoples R China
基金
中国国家自然科学基金;
关键词
Interaction; Besifloxacin; Bovine serum albumin; Spectroscopic techniques; Circular dichroism spectrum; FLUORESCENCE SPECTROSCOPY; HYDROCHLORIDE; BINDING; OXYGEN; MODEL;
D O I
10.1016/j.saa.2015.04.023
中图分类号
O433 [光谱学];
学科分类号
0703 ; 070302 ;
摘要
The interaction between Besifloxacin (BFIX) and bovine serum albumin (BSA) was investigated by spectroscopic (fluorescence, UV-Vis absorption and circular dichroism) techniques under imitated physiological conditions. The experiments were conducted at different temperatures (298, 304 and 310 K) and the results showed that the BFLX caused the fluorescence quenching of BSA through a static quenching procedure. The binding constant (K-a), binding sites (n) were obtained. The corresponding thermodynamic parameters.(Delta H, Delta S and Delta G) of the interaction system were calculated at different temperatures. The results revealed that the binding process was spontaneous and the acting force between BFLX and BSA were mainly electrostatic forces. According to Forster non-radiation energy transfer theory, the binding distance between BFLX and BSA was calculated to be 4.96 nm. What is more, both synchronous fluorescence and circular dichroism spectra confirmed conformational changes of BSA. (C) 2015 Elsevier B.V. All rights reserved.
引用
收藏
页码:116 / 121
页数:6
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