Physical and genetic map of the Finegoldia magna (formerly Peptostreptococcus magnus) ATCC 29328 genome

A physical and genetic map of Finegoldia magna (formerly Peptostreptococcus magnus) ATCC 29328 chromosome was constructed. The order of rare cleavage restriction fragments was determined by double digestion with the restriction enzymes I-CeuI, SgrAI, ApaI and PmeI, cross-hybridization and ApaI-linking clones. The size of the circular chromosome of F magna was estimated to be 1.9 Mb. This strain also had a 200-kb megaplasmid. The chromosome contained four rrn operons. and the orientation of two rrn operons was opposite to the others, Fragment analysis of Peptostreptococcus anaerobius ATCC 27337(T) chromosome suggested that its size was much smaller than that of F magna ATCC 29328. (C) 2002 Published by Elsevier Science B.V. on behalf of the Federation of European Microbiological Societies.