A microwave-assisted solution combustion synthesis to produce europium-doped calcium phosphate nanowhiskers for bioimaging applications

被引:69
|
作者
Wagner, Darcy E. [1 ,2 ]
Eisenmann, Kathryn M. [3 ]
Nestor-Kalinoski, Andrea L. [4 ]
Bhaduri, Sarit B. [5 ]
机构
[1] Univ Toledo, Coll Engn, Dept Biomed Engn, Toledo, OH 43606 USA
[2] Univ Toledo, Coll Med & Life Sci, Dept Biomed Engn, Toledo, OH 43606 USA
[3] Univ Toledo, Coll Med & Life Sci, Dept Biochem & Canc Biol, Toledo, OH 43614 USA
[4] Univ Toledo, Coll Med & Life Sci, Dept Surg, Toledo, OH 43614 USA
[5] Univ Toledo, Coll Engn, Dept Mech Ind & Mfg Engn, Toledo, OH 43606 USA
基金
美国国家科学基金会;
关键词
Europium; Calcium phosphate; Fluorescence; Inorganic nanoparticles; Microwave-assisted solution synthesis; QUANTUM DOTS; HYDROXYAPATITE NANORODS; DRUG-DELIVERY; BIOLOGICAL APPLICATIONS; SILICA NANOPARTICLES; RARE-EARTH; EU3+ IONS; IN-VIVO; LUMINESCENCE; SIZE;
D O I
10.1016/j.actbio.2013.05.033
中图分类号
R318 [生物医学工程];
学科分类号
0831 ;
摘要
Biocompatible nanoparticles possessing fluorescent properties offer attractive possibilities for multifunctional bioimaging and/or drug and gene delivery applications. Many of the limitations with current imaging systems center on the properties of the optical probes in relation to equipment technical capabilities. Here we introduce a novel high aspect ratio and highly crystalline europium-doped calcium phosphate nanowhisker produced using a simple microwave-assisted solution combustion synthesis method for use as a multifunctional bioimaging probe. X-ray diffraction confirmed the material phase as europium-doped hydroxyapatite. Fluorescence emission and excitation spectra and their corresponding peaks were identified using spectrofluorimetry and validated with fluorescence, confocal and multiphoton microscopy. The nanowhiskers were found to exhibit red and far red wavelength fluorescence under ultraviolet excitation with an optimal peak emission of 696 nm achieved with a 350 nm excitation. Relatively narrow emission bands were observed, which may permit their use in multicolor imaging applications. Confocal and multiphoton microscopy confirmed that the nanoparticles provide sufficient intensity to be utilized in imaging applications. (C) 2013 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.
引用
收藏
页码:8422 / 8432
页数:11
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