One hundred and one new microsatellite loci derived from ESTs (EST-SSRs) in bread wheat

被引：137
作者：
Gao, LF
Jing, RL
Huo, NX
Li, Y
Li, XP
Zhou, RH
Chang, XP
Tang, JF
Ma, ZY
Jia, JZ [1 ]
机构：
[1] Chinese Acad Agr Sci, Inst Crop Germplasm Resources, Minist Agr, Key Lab Crop Germplasm & Biotechnol, Beijing 100081, Peoples R China
[2] Heibei Agr Univ, Dept Agron, Baoding 171001, Peoples R China
[3] NW Sci Tech Univ Agr & Forestry, Yang Ling 712100, Peoples R China
来源：
THEORETICAL AND APPLIED GENETICS | 2004年 / 108卷 / 07期
关键词：
D O I：
10.1007/s00122-003-1554-z
中图分类号：
S3 [农学（农艺学）];
学科分类号：
0901 ;
摘要：
Four hundred and seventy-eight microsatellite markers derived from expressed sequence tags (EST-SSRs) were screened among three mapping populations (W-7984xOpata 85, WOpop; LumaixHanxuan, LHpop; WenmaixShanhongmai, WSpop). The number of polymorphic EST-SSR primer pairs found in WOpop, LHpop and WSpop was 92, 58 and 29 respectively. A total of 101 EST-SSR loci amplified from 88 primer sets were distributed over the 20 chromosomes of the reference maps (no markers were located on chromosome 4B). These 101 mapped EST-SSR markers add to the existing 450 microsatellite loci previously mapped in bread wheat. Seventy-four of the 101 loci showed significant similarities to known genes, including 24 genes involved in metabolism, 4 in cellular structures, 9 in stress resistance, 12 in transcription, 2 in development, 2 transporters and 21 storage proteins. Besides gliadin and glutenin, most of the 53 genes with putative functions were mapped for the first time by EST-SSR markers in bread wheat. Sequence alignment of the mapped wheat EST-SSR loci allowed tentative assignment of functionality to the other members of grasses family. Colinearity combined with homology information offers an attractive approach to comparative genomics.
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页码：1392 / 1400
页数：9
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