Cytotoxic and apoptogenic properties of three isolated diterpenoids from Salvia chorassanica through bioassay-guided fractionation

被引：43

作者：

Tayarani-Najaran, Zahra ^{[1
]}

Mousavi, Seyed Hadi ^{[2
,3
]}

Tajfard, Faezeh ^{[1
]}

Asili, Javad ^{[4
]}

Soltani, Saba ^{[5
]}

Hatamipour, Mandi ^{[4
]}

Emami, Seyed Ahmad ^{[4
]}

机构：

[1] Mashhad Univ Med Sci, Sch Pharm, Dept Pharmacodynam & Toxicol, Mashhad, Iran

[2] Mashhad Univ Med Sci, Sch Med, Med Toxicol Res Ctr, Mashhad, Iran

[3] Mashhad Univ Med Sci, Sch Med, Pharmacol Res Ctr Med Plants, Mashhad, Iran

[4] Mashhad Univ Med Sci, Sch Pharm, Dept Pharmacognosy, Mashhad, Iran

[5] North Khorasan Univ Med Sci, Res Ctr Nat Prod Safety & Med Plants, Bojnurd 0183049504, Iran

来源：

FOOD AND CHEMICAL TOXICOLOGY | 2013年 / 57卷

关键词：

Salvia chorassanica; Leukemic cell lines; Apoptosis; Taxodione; Ferruginol; 6-Hydroxysalvinolone; ABIETANE DITERPENES; CELL-LINES; APOPTOSIS; OFFICINALIS; ACTIVATION; ROOTS;

D O I：

10.1016/j.fct.2013.03.037

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

Bioassay-guided fractionation of Salvia chorassanica Bunge roots for the first time led to the isolation of taxodione, ferruginol and 6-hydroxysalvinolone. Cytotoxic studies revealed that all compounds exhibited high cytotoxic activity against apoptosis-proficient HL-60 and apoptosis-resistant K562 cells, with IC50 values ranging from 7.7 to 60.3 mu M with much less cytotoxic effects on normal human lymphocytes. Sub-G1 peak in flow cytometry histogram of treated cells by S. chorassanica and all pure compounds in comparison with control was induced and DNA fragmentation confirmed the cytotoxicity and apoptosis. Taxodione, ferruginol and 6-hydroxysalvinolone (2.5 and 5.0 mu M) also enhanced expression of the proapoptotic protein Bax, cleaved caspase-3 and cleaved PARP after treatment for 48 h. Collectively, these findings suggest cytotoxic and apoptotic effectiveness of taxodione, ferruginol and 6-hydroxysalvinolone against the leukemic K562 and HL-60 cancer cells. (C) 2013 Elsevier Ltd. All rights reserved.

引用

页码：346 / 351

页数：6

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