Immobilization of laccase onto micro-emulsified magnetic nanoparticles for enhanced degradation of a textile recalcitrant

The present investigation evaluates the efficiency of laccase binding onto the micro-emulsified magnetic nanoparticles (MNPs). The synthesized MNPs were characterized for its nature, particle size and functional moieties using transmission electron microscopy Fourier transform infrared spectroscopy and X-ray diffraction studies. It was found that the MNPs had a uniform and mean average diameter of 12.63 +/- 3.2 nm. The TEM analysis and XRD patterns also confirmed the presence of MNPs after laccase binding. The maximum activity of the immobilized laccase was obtained at its weight ratio of 19.55x10(-3) mg bound laccase mg(-1) MNPs. The stability and reusability analyses were also studied to evaluate the activity of the immobilized laccase with that of free laccase. Application of laccase immobilized MNPs were extended to degrade a reactive textile dye; reactive green 19A (RG19A).