Paenibacillus larvae-Directed Bacteriophage HB10c2 and Its Application in American Foulbrood-Affected Honey Bee Larvae

被引：40

作者：

Beims, Hannes ^{[1
]}

Wittmann, Johannes ^{[2
]}

Bunk, Boyke ^{[2
]}

Sproeer, Cathrin ^{[2
]}

Rohde, Christine ^{[2
]}

Guenther, Gabi ^{[1
]}

Rohde, Manfred ^{[3
]}

von der Ohe, Werner ^{[4
]}

Steinert, Michael ^{[1
,3
]}

机构：

[1] Tech Univ Carolo Wilhelmina Braunschweig, Inst Mikrobiol, D-38106 Braunschweig, Germany

[2] Leibniz Inst DSMZ German Collect Microorganisms &, Braunschweig, Germany

[3] Helmholtz Ctr Infect Res, Cent Facil Microscopy, Braunschweig, Germany

[4] Inst Apiculture, Lower Saxony State Off Consumer Protect & Food Sa, Celle, Germany

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2015年 / 81卷 / 16期

关键词：

LYSIS; SEQUENCE; THERAPY; GROWTH; PHAGES;

D O I：

10.1128/AEM.00804-15

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Paenibacillus larvae is the causative agent of American foulbrood (AFB), the most serious honey bee brood bacterial disease. We isolated and characterized P. larvae-directed bacteriophages and developed criteria for safe phage therapy. Whole-genome analysis of a highly lytic virus of the family Siphoviridae (HB10c2) provided a detailed safety profile and uncovered its lysogenic nature and a putative beta-lactamase-like protein. To rate its antagonistic activity against the pathogens targeted and to specify potentially harmful effects on the bee population and the environment, P. larvae genotypes ERIC I to IV, representatives of the bee gut microbiota, and a broad panel of members of the order Bacillales were analyzed for phage HB10c2-induced lysis. Breeding assays with infected bee larvae revealed that the in vitro phage activity observed was not predictive of the real-life scenario and therapeutic efficacy. On the basis of the disclosed P. larvae-bacteriophage coevolution, we discuss the future prospects of AFB phage therapy.

引用

页码：5411 / 5419

页数：9

共 38 条

[1]

Abedon Stephen T, 2011, Bacteriophage, V1, P66

[2]

[Anonymous], 2013, BACTERIOPHAGE

[3]

Aupinel Pierrick, 2005, Bulletin of Insectology, V58, P107

[4]

Boulanger P, 2009, BACTERIOPHAGES METHO, V2

[5] Phages and the evolution of bacterial pathogens:: From genomic rearrangements to lysogenic conversion [J].

Brüssow, H ;

Canchaya, C ;

Hardt, WD .

MICROBIOLOGY AND MOLECULAR BIOLOGY REVIEWS, 2004, 68 (03) :560-+

[6] The generalized transducing Salmonella bacteriophage ES18:: Complete genome sequence and DNA packaging strategy [J].

Casjens, SR ;

Gilcrease, EB ;

Winn-Stapley, DA ;

Schicklmaier, P ;

Schmieger, H ;

Pedulla, ML ;

Ford, ME ;

Houtz, JM ;

Hatfull, GF ;

Hendrix, RW .

JOURNAL OF BACTERIOLOGY, 2005, 187 (03) :1091-1104

[7]

Chan BK, 2013, FUTURE MICROBIOL, V8, P769, DOI [10.2217/fmb.13.47, 10.2217/FMB.13.47]

[8] Updated genome assembly and annotation of Paenibacillus larvae, the agent of American foulbrood disease of honey bees [J].

Chan, Queenie W. T. ;

Cornman, R. Scott ;

Birol, Inanc ;

Liao, Nancy Y. ;

Chan, Simon K. ;

Docking, T. Roderick ;

Jackman, Shaun D. ;

Taylor, Greg A. ;

Jones, Steven J. M. ;

de Graaf, Dirk C. ;

Evans, Jay D. ;

Foster, Leonard J. .

BMC GENOMICS, 2011, 12

[9] How to Kill the Honey Bee Larva: Genomic Potential and Virulence Mechanisms of Paenibacillus larvae [J].

Djukic, Marvin ;

Brzuszkiewicz, Elzbieta ;

Fuenfhaus, Anne ;

Voss, Joern ;

Gollnow, Kathleen ;

Poppinga, Lena ;

Liesegang, Heiko ;

Garcia-Gonzalez, Eva ;

Genersch, Elke ;

Daniel, Rolf .

PLOS ONE, 2014, 9 (03)

[10] Reclassification of Paenibacillus larvae subsp pulvifaciens and Paenibacillus larvae subsp larvae as Paenibacillus larvae without subspecies differentiation [J].

Genersch, E ;

Forsgren, E ;

Pentikäinen, J ;

Ashiralieva, A ;

Rauch, S ;

Kilwinski, J ;

Fries, I .

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 2006, 56 :501-511

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