Modeling and correction of Raman and Rayleigh scatter in fluorescence landscapes

被引：25

作者：

Eilers, Paul H. C. ^{[1
]}

Kroonenberg, Pieter M. ^{[2
]}

机构：

[1] Erasmus Univ, Med Ctr, Rotterdam, Netherlands

[2] Leiden Univ, Dept Child & Family Studies, Leiden, Netherlands

来源：

CHEMOMETRICS AND INTELLIGENT LABORATORY SYSTEMS | 2014年 / 130卷

关键词：

Smoothing; Background subtraction; Rayleigh scatter; Raman scatter; Fluorescence excitation-emission spectroscopy;

D O I：

10.1016/j.chemolab.2013.09.002

中图分类号：

TP [自动化技术、计算机技术];

学科分类号：

0812 ;

摘要：

Rayleigh and Raman scatter in fluorescence (excitation-emission) landscapes are a nuisance in two-way and three-way data modeling. We provide a method to clean individual emission spectra. The scatter can be represented accurately by Gaussian peaks, characterized by location, width and height. The analytic signal of interest effectively acts as a background to the scatter peaks. Modeling it locally as a smooth curve, using penalized least squares, allows accurate estimation of the parameters of scatter peaks. Once the peaks are modeled, they can be subtracted from the spectrum, almost completely removing the artifacts. Apart from local smoothness, no assumptions are made about the fluorescence spectra. (C) 2013 Elsevier B.V. All rights reserved.

引用

页码：1 / 5

页数：5

共 18 条

[1] Practical aspects of PARAFAC modeling of fluorescence excitation-emission data [J].