High-quality reference genes for quantifying the transcriptional responses of Oryza sativa L. (ssp indica and japonica) to abiotic stress conditions

被引:31
|
作者
Maksup, Sarunyaporn [1 ]
Supaibulwatana, Kanyaratt [1 ]
Selvaraj, Gopalan [2 ]
机构
[1] Mahidol Univ, Fac Sci, Dept Biotechnol, Bangkok 10400, Thailand
[2] Natl Res Council Canada, Inst Plant Biotechnol, Saskatoon, SK S7N 0W9, Canada
来源
CHINESE SCIENCE BULLETIN | 2013年 / 58卷 / 16期
关键词
KDML105; microarrays; quantitative real-time PCR; reference gene; rice (Oryza saliva L.); stress responsive gene; TIME RT-PCR; TREHALOSE BIOSYNTHESIS; EXPRESSION ANALYSIS; HOUSEKEEPING GENES; PHOSPHATASE GENE; INTERNAL CONTROL; RICE; NORMALIZATION; IDENTIFICATION; ARABIDOPSIS;
D O I
10.1007/s11434-013-5726-1
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Rice (Oryza saliva L.) is important to food security and is also an excellent model plant for numerous cereal crops. A functional genomics study in rice includes characterization of the expression dynamics of genes by quantitative real-time PCR (qPCR) analysis; this is a significant key for developing rice varieties that perform well in the face of adverse climate change. The qPCR analysis requires the use of appropriate reference genes in order to make any quantitative interpretations meaningful. Here, the new potential reference genes were selected from a huge public database of rice microarray experiments. The expression stability of 14 candidates and 4 conventional reference genes was validated by geNorm(PLUS) and NormFinder software. Seven candidates are superior to the conventionally used reference genes in qPCR and three genes can be used reliably for quantitating the expression of genes involved in abiotic stress responses. These high-quality references EP (LOC_Os05g08980), HNR (LOC_Os01g71770), and TBC (LOC_Os09g34040) worked very well in three indica genotypes and one japonica genotype. One of indica genotypes including the Jasmine rice, KDML105 developed in Thailand for which no reference genes have been reported until now.
引用
收藏
页码:1919 / 1930
页数:12
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