RNA polymerase II (Pol II) elongation in metazoans is thought to require phosphorylation of serine 2 (Ser2-P) of the Pol II C-terminal domain (CTD) by the P-TEFb complex, CDK-9/cyclin T. Another Ser2 kinase complex, CDK-12/cyclin K, which requires upstream CDK-9 activity has been identified in Drosophila and human cells. We show that regulation of Ser2-P in C. elegans soma is similar to other metazoan systems, but Ser2-P in the germline is independent of CDK-9, and largely requires only CDK-12. The observed differences are not due to differential tissue expression as both kinases and their cyclin partners are ubiquitously expressed. Surprisingly, loss of CDK-9 from germ cells has little effect on Ser2-P, yet CDK-9 is essential for germline development. By contrast, loss of CDK-12 and Ser2-P specifically from germ cells has little impact on germline development or function, although significant loss of co-transcriptional H3K36 trimethylation is observed. These results show a reduced requirement for Pol II Ser2-P in germline development and suggest that generating Ser2-P is not the essential role of CDK-9 in these cells. Transcriptional elongation in the C. elegans germline thus appears to be uniquely regulated, which may be a novel facet of germline identity.
机构:
Univ Calif San Francisco, Dept Med, San Francisco, CA 94143 USA
Univ Calif San Francisco, Dept Microbiol, San Francisco, CA 94143 USA
Univ Calif San Francisco, Dept Immunol, San Francisco, CA 94143 USA
Dept Med, San Francisco, CA 94143 USAUniv Calif San Francisco, Dept Med, San Francisco, CA 94143 USA
Huang, Fang
Nguyen, Trang T. T.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Calif San Francisco, Dept Med, San Francisco, CA 94143 USA
Univ Calif San Francisco, Dept Microbiol, San Francisco, CA 94143 USA
Univ Calif San Francisco, Dept Immunol, San Francisco, CA 94143 USA
Dept Med, San Francisco, CA 94143 USA
Howard Hughes Med Inst, San Francisco, CA USAUniv Calif San Francisco, Dept Med, San Francisco, CA 94143 USA
Nguyen, Trang T. T.
Echeverria, Ignacia
论文数: 0引用数: 0
h-index: 0
机构:
Univ Calif San Francisco, Dept Bioengn & Therapeut Sci, San Francisco, CA 94143 USA
Calif Inst Quantitat Biosci QBI, Dept Cellular Mol Pharmacol, San Francisco, CA USA
Dept Bioengn & Therapeut Sci, San Francisco, CA USAUniv Calif San Francisco, Dept Med, San Francisco, CA 94143 USA
Echeverria, Ignacia
Ramachandran, Rakesh
论文数: 0引用数: 0
h-index: 0
机构:
Univ Calif San Francisco, Dept Bioengn & Therapeut Sci, San Francisco, CA 94143 USA
Calif Inst Quantitat Biosci QBI, Dept Cellular Mol Pharmacol, San Francisco, CA USA
Dept Bioengn & Therapeut Sci, San Francisco, CA USAUniv Calif San Francisco, Dept Med, San Francisco, CA 94143 USA
Ramachandran, Rakesh
Cary, Daniele C.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Calif San Francisco, Dept Med, San Francisco, CA 94143 USA
Univ Calif San Francisco, Dept Microbiol, San Francisco, CA 94143 USA
Univ Calif San Francisco, Dept Immunol, San Francisco, CA 94143 USA
Dept Med, San Francisco, CA 94143 USAUniv Calif San Francisco, Dept Med, San Francisco, CA 94143 USA
Cary, Daniele C.
Paculova, Hana
论文数: 0引用数: 0
h-index: 0
机构:
Univ Calif San Francisco, Dept Med, San Francisco, CA 94143 USA
Univ Calif San Francisco, Dept Microbiol, San Francisco, CA 94143 USA
Univ Calif San Francisco, Dept Immunol, San Francisco, CA 94143 USAUniv Calif San Francisco, Dept Med, San Francisco, CA 94143 USA
Paculova, Hana
Sali, Andrej
论文数: 0引用数: 0
h-index: 0
机构:
Univ Calif San Francisco, Dept Bioengn & Therapeut Sci, San Francisco, CA 94143 USA
Calif Inst Quantitat Biosci QBI, Dept Bioengn & Therapeut Sci, Dept Pharmaceut Chem, San Francisco, CA USA
Calif Inst Quantitat Biosci QBI, San Francisco, CA USAUniv Calif San Francisco, Dept Med, San Francisco, CA 94143 USA
Sali, Andrej
Weiss, Arthur
论文数: 0引用数: 0
h-index: 0
机构:
Univ Calif San Francisco, Dept Med, San Francisco, CA 94143 USA
Univ Calif San Francisco, Dept Microbiol, San Francisco, CA 94143 USA
Univ Calif San Francisco, Dept Immunol, San Francisco, CA 94143 USA
Dept Med, San Francisco, CA 94143 USA
Howard Hughes Med Inst, San Francisco, CA USAUniv Calif San Francisco, Dept Med, San Francisco, CA 94143 USA
Weiss, Arthur
Peterlin, Boris Matija
论文数: 0引用数: 0
h-index: 0
机构:
Univ Calif San Francisco, Dept Med, San Francisco, CA 94143 USA
Univ Calif San Francisco, Dept Microbiol, San Francisco, CA 94143 USA
Univ Calif San Francisco, Dept Immunol, San Francisco, CA 94143 USA
Dept Med, San Francisco, CA 94143 USAUniv Calif San Francisco, Dept Med, San Francisco, CA 94143 USA
Peterlin, Boris Matija
Fujinaga, Koh
论文数: 0引用数: 0
h-index: 0
机构:
Univ Calif San Francisco, Dept Med, San Francisco, CA 94143 USA
Univ Calif San Francisco, Dept Microbiol, San Francisco, CA 94143 USA
Univ Calif San Francisco, Dept Immunol, San Francisco, CA 94143 USA
Dept Med, San Francisco, CA 94143 USAUniv Calif San Francisco, Dept Med, San Francisco, CA 94143 USA
机构:Univ Med & Dent New Jersey, Robert Wood Johnson Med Sch, Dept Pharmacol, Piscataway, NJ 08854 USA
Ping, YH
Rana, TM
论文数: 0引用数: 0
h-index: 0
机构:
Univ Med & Dent New Jersey, Robert Wood Johnson Med Sch, Dept Pharmacol, Piscataway, NJ 08854 USAUniv Med & Dent New Jersey, Robert Wood Johnson Med Sch, Dept Pharmacol, Piscataway, NJ 08854 USA