Rapid determination of nevirapine in human plasma by gas chromatography

被引：14

作者：

Langmann, P ^{[1
]}

Schirmer, D ^{[1
]}

Väth, T ^{[1
]}

Desch, S ^{[1
]}

Zilly, M ^{[1
]}

Klinker, H ^{[1
]}

机构：

[1] Univ Wurzburg, Dept Internal Med, Div Infect Dis & Hepatol, D-97080 Wurzburg, Germany

来源：

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2002年 / 767卷 / 01期

关键词：

nevirapine;

D O I：

10.1016/S0378-4347(01)00528-X

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A sensitive and rapid gas chromatographic method has been developed to determine the levels of the HIV-1 non-nucleoside reverse transcriptase inhibitor nevirapine in human plasma. Quantitative recovery following liquid-liquid-extraction with diethylether from 500 mul of human plasma was achieved. Subsequently, the assay was performed with a CP-Sil 5CB capillary column, 15 mX0.32 mmX1.0 mum film thickness with a nitrogen-phosphorous-detector (NPD), Helium 5.0 was used as carrier gas with a constant inlet pressure of 7 p.s.i. Linear standard curves were obtained for concentrations ranging from 10 to 20 000 ng/ml. The calculated intra- and inter-day coefficients of variation were below 8%. (C) 2002 Elsevier Science B.V. All rights reserved.

引用

页码：69 / 74

页数：6

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