Inhibitory Effects of Echinochrome A, Isolated from Shell of the Sea Urchin Anthocidaris crassispina, on Antigen-Stimulated Degranulation in Rat Basophilic Leukemia RBL-2H3 Cells through Suppression of Lyn Activation

被引：0

作者：

Itoh, Tomohiro ^{[1
,2
]}

Fujiwara, Azusa ^{[2
]}

Ninomiya, Masayuki ^{[3
]}

Maeda, Toshimichi ^{[4
]}

Ando, Masashi ^{[2
]}

Tsukamasa, Yasuyuki ^{[2
]}

Koketsu, Mamoru ^{[3
]}

机构：

[1] Mie Univ, Grad Sch Bioresources, Dept Life Sci, Lab Mol Chem Aquat Mat, 1577 Kurimamachiya, Tsu, Mie 5148507, Japan

[2] Kinki Univ, Fac Agr, Dept Fisheries, Lab Aquat Food Sci, 3327-204 Nakamachi, Nara 6318505, Japan

[3] Gifu Univ, Fac Engn, Dept Chem & Biomol Sci, 1-1 Yanagido, Gifu 5011193, Japan

[4] Natl Fisheries Univ, Grad Sch Fisheries Sci Food Sci & Technol, 2-7-1 Nagata Honmachi, Shimonoseki, Yamaguchi 7596595, Japan

来源：

NATURAL PRODUCT COMMUNICATIONS | 2016年 / 11卷 / 09期

关键词：

Sea urchin; Echinochrome A; Mast cell degranulation; Type I allergy; Lyn; PIGMENT ECHINOCHROME;

D O I：

暂无

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

Echinochrome A (Echi-A) was isolated from the sea urchin Anthocidaris crassispina and its structure determined using 1D and 2D-NMR. In the present study, we examined the inhibitory effect of Echi-A on antigen -stimulated degranulation in rat basophilic leukemia RBL-2H3 cells, which were suppressed in a dose dependent manner. The antigens bind to the high affinity immunoglobulin E receptor, which is expressed on the surface of mast cells and basophils and activate intracellular signal transduction, resulting in the release of biologically active mediators such as histamine. In order to disclose the inhibitory mechanisms of degranulation by Echi-A, we examined the elevation in intracellular Ca2+ concentration ([Ca2+]), production levels of intracellular reactive oxygen species (ROS) and early intracellular signaling events. Both elevation of [Ca2+], and intracellular ROS production were markedly suppressed in cells treated with Echi-A. Echi-A also suppressed the activation of Lyn, Syk, and PLC gamma 1/2 in antigenstimulated cells. These results indicated that inhibition of antigen-stimulated degranulation in RBL-2H3 cells by Echi-A is mainly due to the inactivation of Lyn/Syk/PLC gamma signaling pathways. Our findings suggest that Echi-A could be a beneficial agent for alleviating the symptoms of type I allergy.

引用

页码：1303 / 1306

页数：4