The Requirement for Carotenoids in the Assembly and Function of the Photosynthetic Complexes in Chlamydomonas reinhardtii

被引：33

作者：

Santabarbara, Stefano ^{[1
,2
,5
]}

Casazza, Anna Paola ^{[3
]}

Ali, Kulsam ^{[1
]}

Economou, Chloe K. ^{[1
]}

Wannathong, Thanyanun ^{[1
]}

Zito, Francesca ^{[4
]}

Redding, Kevin E. ^{[6
]}

Rappaport, Fabrice ^{[5
]}

Purton, Saul ^{[1
]}

机构：

[1] UCL, Inst Struct & Mol Biol, London WC1E 6BT, England

[2] CNR, Ist Biofis, I-20133 Milan, Italy

[3] CNR, Inst Biol & Biotecnol Agr, I-20133 Milan, Italy

[4] Univ Paris 07, CNRS, UMR 7099, F-75005 Paris, France

[5] Univ Paris 06, CNRS, UMR 7141, Inst Biol Physicochim, F-75005 Paris, France

[6] Arizona State Univ, Dept Chem & Biochem, Tempe, AZ 85287 USA

来源：

PLANT PHYSIOLOGY | 2013年 / 161卷 / 01期

基金：

英国生物技术与生命科学研究理事会;

关键词：

BIDIRECTIONAL ELECTRON-TRANSFER; REACTION-CENTER SUBUNITS; PHOTOSYSTEM-I; ENERGY-TRANSFER; CYTOCHROME B(6)F; TEMPERATURE-DEPENDENCE; CRYSTAL-STRUCTURE; SINGLET OXYGEN; BETA-CAROTENE; CHLOROPHYLL-A;

D O I：

10.1104/pp.112.205260

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

We have investigated the importance of carotenoids on the accumulation and function of the photosynthetic apparatus using a mutant of the green alga Chlamydomonas reinhardtii lacking carotenoids. The FN68 mutant is deficient in phytoene synthase, the first enzyme of the carotenoid biosynthesis pathway, and therefore is unable to synthesize any carotenes and xanthophylls. We find that FN68 is unable to accumulate the light-harvesting complexes associated with both photosystems as well as the RC subunits of photosystem II. The accumulation of the cytochrome b(6)f complex is also strongly reduced to a level approximately 10% that of the wild type. However, the residual fraction of assembled cytochrome b6f complexes exhibits single-turnover electron transfer kinetics comparable to those observed in the wild-type strain. Surprisingly, photosystem I is assembled to significant levels in the absence of carotenoids in FN68 and possesses functional properties that are very similar to those of the wild-type complex.

引用

页码：535 / 546

页数：12

共 66 条

[1] Temperature dependence of biphasic forward electron transfer from the phylloquinone(s) A1 in photosystem I:: only the slower phase is activated [J].