Age dependent effects of NELL-1 isoforms on bone marrow stromal cells

被引：6

作者：

Meyers, Carolyn A. ^{[1
]}

Sun, Zhibo ^{[1
]}

Chang, Leslie ^{[1
]}

Ding, Catherine ^{[2
]}

Lu, Amy ^{[1
]}

Ting, Kang ^{[2
]}

Pang, Shen ^{[3
,4
,5
]}

James, Aaron W. ^{[1
,3
,4
,5
]}

机构：

[1] Johns Hopkins Univ, Dept Pathol, Ross Res Bldg,Room 524A,720 Rutland Ave, Baltimore, MD 21205 USA

[2] Univ Calif Los Angeles, Sch Dent, Div Growth & Dev, Los Angeles, CA 90024 USA

[3] UCLA, Los Angeles, CA 90095 USA

[4] Orthoped Hosp, Dept Orthopaed Surg, Los Angeles, CA 90095 USA

[5] Orthoped Hosp, Res Ctr, Los Angeles, CA 90095 USA

来源：

JOURNAL OF ORTHOPAEDICS | 2019年 / 16卷 / 02期

关键词：

Mesenchymal stem cell; Nell-1; BMSC; Osteogenesis; PERIVASCULAR STEM-CELLS; DIFFERENTIATION; EXPRESSION; PROLIFERATION; OSTEOGENESIS; ADHESION; DELIVERY; RUNX2; GENE; MICE;

D O I：

10.1016/j.jor.2019.02.006

中图分类号：

R826.8 [整形外科学]; R782.2 [口腔颌面部整形外科学]; R726.2 [小儿整形外科学]; R62 [整形外科学（修复外科学）];

学科分类号：

摘要：

Objective: NELL-1 is an osteogenic protein first discovered to control ossification of the cranium. NELL-1 exists in at least two isoforms. The full-length NELL-1 contains 810 amino acid (aa) (NELL-1810), the N-terminal-truncated NELL-1 isoform contains 570 aa (NELL-1570). The differences in cellular effects between NELL-1 isoforms are not well understood. Methods: Here, BMSC were derived from adult or aged mice, followed by overexpression of NELL-1810 or NELL-1570. Cell morphology, proliferation, and gene expression were examined. Results/Conclusions: Overall, the proliferative effect of NELL-1570 was age dependent, showing prominent induction in adult but not aged mice.

引用

页码：175 / 178

页数：4

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