Optical device and methodology for optical sensing of hemolysis in hypotonic media

被引:9
作者
Marquez-Islas, Roberto [1 ]
Perez-Pacheco, Argelia [1 ]
Beatriz Salazar-Nieva, Laura [2 ]
Acevedo-Barrera, Anays [3 ]
Mendoza-Garcia, Emma [4 ]
Garcia-Valenzuela, Augusto [3 ]
机构
[1] Hosp Gen Mexico Dr Eduardo Liceaga, Unidad Invest & Desarrollo Tecnol, Dr Balmis 148, Ciudad De Mexico 06726, Mexico
[2] Univ Nacl Autonoma Mexico, Fac Ciencias, Av Univ 3000,Circuito Exterior S-N Alcaldia, Ciudad De Mexico 04510, Mexico
[3] Univ Nacl Autonoma Mexico, Inst Ciencias Aplicadas & Tecnol, Apartado Postal 70-185, Ciudad De Mexico 04510, Mexico
[4] Hosp Gen Mexico Dr Eduardo Liceaga, Lab Hematol Especial, Dr Balmis 148, Ciudad De Mexico 06726, Mexico
关键词
medical optics instrumentation; reflection; sensors; cell analysis; total internal reflection; refractive index measurements; OSMOTIC FRAGILITY; LIGHT-SCATTERING; BLOOD; ERYTHROCYTES; HEMOGLOBIN; BIOFLUID; KINETICS;
D O I
10.1088/1361-6501/ab8430
中图分类号
T [工业技术];
学科分类号
08 ;
摘要
Identifying abnormalities in red blood cells can provide important medical clues for the diagnosis, prognosis, and treatment of some health disorders. A common test used to examine the conditions of erythrocytes in humans is osmotic fragility. The standard technique to determine the osmotic fragility of red blood cells is laborious and time-consuming, and provides only approximate values with a few experimental data. In this work, we propose and investigate a way to measure the osmotic fragility of erythrocytes, rapidly and in a straightforward, more quantitative and less laborious way, with an optical sensor. The basic idea is monitoring in real time the refractive index of a suspension of erythrocytes to follow the kinetics of their lysis as the hemoglobin-rich cytosol is released from the erythrocytes in the surrounding hypotonic solution during hemolysis, resulting in an increase of the refractive index. We propose a device to measure in real time the refractive index and demonstrate the feasibility of monitoring the hemolysis process with high resolution. Our results show that the release of hemoglobin-rich cytosol during the progress of the hemolysis of erythrocytes in hypotonic media is generally an exponential-like function of time. We provide a mathematical model that reproduces experimental curves effectively. We show results for erythrocytes obtained from human blood stored in a blood bank and from volunteers diagnosed with two different types of anemia: hemolytic and drepanocytic. We define times, measurable from the kinetics of the refractive index signal, to quantify the progress of hemolysis as a possible new method of measuring osmotic fragility.
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页数:11
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