Use of polymerase chain reaction for improved diagnosis of tuberculosis in children

被引:0
作者
Tang, TH
Musa, M
Zainuddin, ZF
Choo, KE
Noh, LM
Myat, KA
Yaacob, I
Ismail, H
Mustaffa, M
Musa, M
机构
[1] Univ Sains Malaysia, Sch Med Sci, Kota Bharu, Kelantan, Malaysia
[2] Kota Bharu Hosp, Paediat Unit, Kota Bharu, Kelantan, Malaysia
[3] Kota Bharu Hosp, Chest Clin, Kota Bharu, Kelantan, Malaysia
关键词
B9; IS6110; Mycobacterium tuberculosis; paediatric; PCR;
D O I
暂无
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Tuberculosis (TB) in children is severe especially for those under 5 years old, with a tendency to extrapulmonary spread to other organs with high morbidity and mortality. Thus, in contrast to most adult cases, TB in children often requires urgent treatment but diagnosis is often difficult to establish by clinical and conventional laboratory methods. Rapid tests based on molecular techniques for diagnosis of TB have been widely reported but relatively few involve paediatric cases. In this study, a polymerase chain reaction (PCR)-based test using primers derived from the insertion sequence IS6110 and a new Mycobacterium tuberculosis complex-specific target designated as B9 was developed and used for detecting the organism in specimens taken from four paediatric patients. This method was found to be a useful addition to the current battery of tests available to the paediatrician particularly in cases where diagnoses are difficult to establish.
引用
收藏
页码:47 / 51
页数:5
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