Protective effects of miRNA-589 on cerebral ischemia-reperfusion injury

被引：2

作者：

Ma, G-P ^{[1
]}

Yang, B-Z ^{[2
]}

Zhang, Y-S ^{[1
]}

Wang, B. ^{[1
]}

Wei, X-H ^{[1
]}

Zhang, R-F ^{[1
]}

Jia, K-H ^{[1
]}

Gao, J-P ^{[1
]}

机构：

[1] First Hosp Tianshui, Dept Neurol, 105 Jianshe Rd, Tianshui 741000, Gansu, Peoples R China

[2] First Hosp Tianshui, Dept Special Needs Diag & Treatment, Tianshui, Peoples R China

来源：

JOURNAL OF BIOLOGICAL REGULATORS AND HOMEOSTATIC AGENTS | 2020年 / 34卷 / 04期

关键词：

MiRNA-589; cerebral IRI; BV2; TRAF6; inflammation; BRAIN; INFLAMMATION; STROKE; BLOOD; EXPRESSION; MICRORNA; TRAF6;

D O I：

暂无

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

To uncover the potential influence of microRNA-589 (miRNA-589) on cerebral ischemia-reperfusion injury (IRI) and the underlying mechanism, BV2 cells were stimulated by lipopolysaccharide (LPS) or conditioned medium (CM) of primary cortical neurons undergoing oxygen-glucose deprivation (OGD). Regulatory effects of miRNA-589 on the release of inflammatory factors in BV2 cells induced with LPS or CM of primary cortical neurons undergoing OGD were detected by quantitative real-time polymerase chain reaction (qRT-PCR) and enzyme-linked immunosorbent assay (ELISA). The interaction between miRNA-589 and TRAF6 was finally assessed by dual-luciferase reporter gene assay. MiRNA-589 was downregulated in BV2 cells induced with LPS or CM of primary cortical neurons undergoing OGD. Overexpression of miRNA-589 reduced the release of inflammatory factors in LPS or CM-induced BV2 cells. TRAF6 was verified to be the downstream gene of miRNA-589, and its level was negatively regulated by miRNA-589. MiRNA-589 is downregulated following cerebral IRI and alleviates inflammatory response through negatively regulating TRAF6.

引用

页码：1269 / 1275

页数：7

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