Novel creatine biosensors based on all solid-state contact ammonium-selective membrane electrodes

被引：4

作者：

Altikatoglu, Melda ^{[1
]}

Karakus, Emine ^{[1
]}

Erci, Vildan ^{[2
]}

Pekyardimci, Sule ^{[3
]}

Isildak, Ibrahim ^{[2
]}

机构：

[1] Yildiz Tech Univ, Fac Arts & Sci, Dept Chem, TR-34210 Istanbul, Turkey

[2] Yildiz Tech Univ, Fac Chem & Met Engn, Dept Bioengn, TR-34210 Istanbul, Turkey

[3] Ankara Univ, Fac Sci, Dept Chem, TR-06100 Ankara, Turkey

来源：

ARTIFICIAL CELLS NANOMEDICINE AND BIOTECHNOLOGY | 2013年 / 41卷 / 02期

关键词：

Creatine; all solid-state biosensors; potentiometry; carboxylated PVC; palmitic acid; POTENTIOMETRIC BIOSENSORS; FLOW-INJECTION; PVC MEMBRANE; URIC-ACID; UREA; SYSTEM; PH;

D O I：

10.3109/10731199.2012.696066

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Novel creatine bienzymatic potentiometric biosensors were prepared by immobilizing urease and creatinase on all solid-state contact PVC-containing palmitic acid and carboxylated PVC matrix membrane ammonium-selective electrodes without inner reference solution. Potentiometric characteristics of biosensors were examined in physiological model solutions at different creatine concentrations. The linear working range and long-term sensitivity of the biosensors were also determined. The creatine biosensors prepared by using the carboxylated PVC membrane electrodes showed more effective performance than those of the PVC containing palmitic acid membrane electrodes. Creatine assay in serum samples was successfully carried out by using the standard addition method.

引用

页码：131 / 136

页数：6

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