Visualizing the localization of transfection complexes during graphene nanoparticle-based transfection

被引：2

作者：

ten Bruggencate, Filip ^{[1
]}

Laroche, Fabrice ^{[1
]}

Zhang, Yue ^{[2
]}

Song, Guoqiang ^{[2
]}

Yin, Shougen ^{[3
,4
]}

Abrahams, Jan Pieter ^{[1
]}

Liu, Zunfeng ^{[1
,2
]}

机构：

[1] Leiden Inst Chem, NL-2333 CC Leiden, Netherlands

[2] Changzhou Univ, Sch Pharmaceut Engn & Life Sci, Changzhou 213164, Peoples R China

[3] Tianjin Univ Technol, Key Lab Display Mat & Photoelect Devices, Minist Educ, Tianjin 300384, Peoples R China

[4] Tianjin Univ Technol, Sch Mat Sci & Engn, Tianjin 300384, Peoples R China

来源：

JOURNAL OF MATERIALS CHEMISTRY B | 2013年 / 1卷 / 46期

基金：

中国国家自然科学基金;

关键词：

PROTON SPONGE; GENE DELIVERY; POLYETHYLENIMINE; VIRUSES;

D O I：

10.1039/c3tb21349h

中图分类号：

TB3 [工程材料学]; R318.08 [生物材料学];

学科分类号：

0805 ; 080501 ; 080502 ;

摘要：

Recent research has shown that ultra-small graphene oxide (USGO) particles functionalized with polyethylenimine (PEI) can be used to transfect human cell lines with very high efficiency and low toxicity. In this study, confocal fluorescence microscopy is used to provide more insight into the nature of the complexes formed by DNA and USGO_PEI and to track their localization during the transfection process. The results indicate that the DNA enters the nucleus in complex with the USGO_PEI, which remains inside the nucleus. Also, it is observed that USGO_PEI and plasmid DNA form large aggregates in the transfection medium. Both observations raise concerns that this type of nanomaterial might need further improvements for therapeutic use.

引用

页码：6353 / 6358

页数：6