Amentoflavone induces cell cycle arrest, apoptosis, and autophagy in BV-2 cells

被引：14

作者：

Liu, Zheng ^{[1
,2
]}

Wang, Feng ^{[1
,2
]}

Ma, Hui ^{[1
]}

Xia, Hechun ^{[1
]}

Tian, Jihui ^{[1
]}

Sun, Tao ^{[1
,2
]}

机构：

[1] Ningxia Med Univ, Dept Neurosurg, Gen Hosp, 804 South Shengli St, Yinchuan 750004, Ningxia, Peoples R China

[2] Ningxia Med Univ, Incubat Base Natl Key Lab, Ningxia Key Lab Cerebrocranial Dis, Yinchuan 750004, Ningxia, Peoples R China

来源：

FRONTIERS IN BIOSCIENCE-LANDMARK | 2020年 / 25卷

基金：

中国国家自然科学基金;

关键词：

Epilepsy; Amentoflavone; BV-2; cells; Apoptosis; Cell cycle arrest; Autophagy; COLORECTAL-CANCER; EPILEPSY; PATHWAY; GROWTH; PROLIFERATION; INFLAMMATION; GENES; PHASE; NEUROPROTECTION; SUPPRESSION;

D O I：

10.2741/4835

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Previous studies have shown that amentoflavone (AF) elicits anti-inflammatory and neuroprotective effects. To further investigate the effects of AF on the microglia cell line BV-2, proteomic analysis was performed to screen potential key regulators. The top 5 canonical pathways associated with AF treatment were EIF2 signaling, regulation of eIF4 and p70s6k signaling, mTOR signaling, protein ubiquitination pathway and phagosome maturation. The top up-regulated genes were DOCK2, SEC23A, ME1, UGGT1 and STOM, while the most down-regulated molecules were IGF2R, ATP5O, DDX47, WBP11 and IKBIP. AF significantly decreased BV-2 cell proliferation. It induced cell cycle arrest at G2/M, increased CDK2, p27Kip1 and p53/p-p53, and decreased CDK1/CDC2 and cyclin B1. Cell apoptosis was induced, with increased levels of BAX, c-caspase-3 and ccaspase-9, and decreased levels of BCL-XL. Increased level of autophagosome induced by AF was observed, and increased Beclin-1 and decreased phosphorylation of PI3K and Erk1 were found as well. In conclusion, AF induces cell cycle arrest at G2/M, promotes apoptosis and autophagy in BV-2 cells, which may account for the anti-inflammatory effect of AF in epilepsy.

引用

页码：798 / 816

页数：19

共 63 条

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