Acanthamoeba castellanii cysts: new ultrastructural findings

被引：14

作者：

Chavez-Munguia, Bibiana ^{[1
]}

Salazar-Villatoro, Lizbeth ^{[1
]}

Lagunes-Guillen, Anel ^{[1
]}

Omana-Molina, Maritza ^{[2
]}

Espinosa-Cantellano, Martha ^{[1
]}

Martinez-Palomo, Adolfo ^{[1
]}

机构：

[1] Ctr Res & Adv Studies, Dept Infect & Mol Pathogenesis, Mexico City 07360, DF, Mexico

[2] UNAM, Fac Super Studies, Iztacala, Tlalnepantla, Mexico

来源：

PARASITOLOGY RESEARCH | 2013年 / 112卷 / 03期

关键词：

ACTIN SYNTHESIS; FINE-STRUCTURE; ENCYSTATION; PROTEIN; IDENTIFICATION; RESISTANCE; EXPRESSION; TURNOVER;

D O I：

10.1007/s00436-012-3261-7

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

During Acanthamoeba castellanii trophozoite-cysts differentiation, four morphological stages were identified by scanning electron microscopy: trophozoite, precyst, immature cysts, and mature cysts. Fluorescence microscopy reveals the presence of small cumulus of actin in the cytoplasm of precysts after treatment with rhodamine phalloidin. By the contrary, in mature cysts, fluorescence was not observed. However, when excystation was induced, large fluorescent patches were present. By transmission electron microscopy, encysting amebas showed small cytoplasmic vesicles containing fibrillar material, surrounded by a narrow area of thin fibrils. Similar appearance was observed in pseudopods and phagocytic invaginations. In addition, large aggregates of rod-shape elements, similar to the chromatoid bodies, described in other amebas, were present in the cytoplasm. These cysts presented large areas with orange fluorescence after treatment with acridine orange.

引用

页码：1125 / 1130

页数：6

共 32 条

[1]

Alafag Joanna It-itan, 2006, Korean Journal of Parasitology, V44, P331, DOI 10.3347/kjp.2006.44.4.331

[2] Acanthamoeba healyi:: Molecular cloning and characterization of a coronin homologue, an actin-related protein [J].

Baldo, ET ;

Moon, EK ;

Kong, HH ;

Chung, DI .

EXPERIMENTAL PARASITOLOGY, 2005, 110 (02) :114-122

[3] RESISTANCE OF CYSTS OF AMEBAS TO MICROBIAL DECOMPOSITION [J].

BARRETT, RA ;

ALEXANDER, M .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1977, 33 (03) :670-674

[4] MOLECULAR-SIZE AND CHAIN-LENGTH DISTRIBUTION IN ACANTHAMOEBA CELLULOSE [J].

BLANTON, WE ;

VILLEMEZ, CL .

JOURNAL OF PROTOZOOLOGY, 1978, 25 (02) :264-267

[5] FINE STRUCTURE OF ACANTHAMOEBA CASTELLANII .1. TROPHOZOITE [J].

BOWERS, B ;

KORN, ED .

JOURNAL OF CELL BIOLOGY, 1968, 39 (01) :95-&

[6]

Carter WW, 2004, AIDS READ, V14, P48

[7]

Castillo-Romero A, 2010, PLOS ONE, V4, P1

[8] Participation of Actin on Giardia lamblia Growth and Encystation [J].

Castillo-Romero, Araceli ;

Leon-Avila, Gloria ;

Perez Rangel, Armando ;

Cortes Zarate, Rafael ;

Garcia Tovar, Carlos ;

Manuel Hernandez, Jose .

PLOS ONE, 2009, 4 (09)

[9] Ultrastructural study of encystation and excystation in Acanthamoeba castellanii [J].

Chávez-Munguía, B ;

Omaña-Molina, M ;

González-Lázaro, M ;

González-Robles, A ;

Bonilla, P ;

Martínez-Palomo, A .

JOURNAL OF EUKARYOTIC MICROBIOLOGY, 2005, 52 (02) :153-158

[10] Acanthamoeba castellanii:: Identification and distribution of actin cytoskeleton [J].

Gonzalez-Robles, Arturo ;

Castanon, Guadalupe ;

Hernandez-Ramirez, Vernica Ivonne ;

Salazar-Villatoro, Lizbeth ;

Gonzalez-Lazaro, Monica ;

Omana-Molina, Maritza ;

Talamas-Rohana, Patricia ;

Martinez-Palomo, Adolfo .

EXPERIMENTAL PARASITOLOGY, 2008, 119 (03) :411-417

← 1 2 3 4 →