SARS-CoV-2 proteome microarray for COVID-19 patient sera profiling

被引：1

作者：

La, Dan-yu ^{[1
]}

Jiang, He-wei ^{[1
]}

Li, Yang ^{[2
]}

Zhang, Hai-nan ^{[1
]}

Tao, Sheng-ce ^{[1
]}

机构：

[1] Shanghai Jiao Tong Univ, Shanghai Ctr Syst Biomed, Key Lab Syst Biomed, Minist Educ, Shanghai, Peoples R China

[2] Nankai Univ, Coll Life Sci, Tianjin 300071, Peoples R China

来源：

STAR PROTOCOLS | 2022年 / 3卷 / 02期

基金：

中国国家自然科学基金;

关键词：

Biotechnology and bioengineering; Clinical Protocol; Health Sciences; Immunology; Protein Biochemistry; Protein expression and purification; Proteomics;

D O I：

10.1016/j.xpro.2022.101238

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The immunogenicity of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) proteome is largely unknown. Here we describe a protocol for analyzing sera samples with SARS-CoV-2 proteome microarray. The proteins were ex-pressed by either E. coli expression system or eukaryotic cell expression systems and obtained by affinity purification. The protocol includes microarray fabri-cating and sera profiling, which will be used to build an antibody response land-scape for IgG and IgM. The protocol may help to facilitate a deeper understand-ing of immunity related to SARS-CoV-2.For complete details on the use and execution of this protocol, please refer to Li et al. (2021c).

引用

页数：13

共 5 条

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