Towards a 3D culture of mouse ovarian follicles

被引：37

作者：

Belli, Martina ^{[1
]}

Vigone, Giulia ^{[1
]}

Merico, Valeria ^{[1
]}

Redi, Carlo Alberto ^{[1
]}

Zuccotti, Maurizio ^{[2
]}

Garagna, Silvia ^{[1
,3
]}

机构：

[1] Univ Pavia, Dipartimento Biol & Biotecnol Lazzaro Spallanzani, Lab Biol Sviluppo, I-27100 Pavia, Italy

[2] Univ Parma, Dipartimento Sci Biomed Biotecnol & Traslaz, I-43125 Parma, Italy

[3] Univ Pavia, Ctr Ingn Tissutale, I-27100 Pavia, Italy

来源：

INTERNATIONAL JOURNAL OF DEVELOPMENTAL BIOLOGY | 2012年 / 56卷 / 10-12期

关键词：

3D culture; ovarian follicle; follicle organization; follicle architecture; matrix; IN-VITRO MATURATION; PRE-ANTRAL FOLLICLES; EXTRACELLULAR-MATRIX; STEM-CELLS; OOCYTE MATURATION; 3-DIMENSIONAL CULTURE; PREANTRAL FOLLICLES; ALGINATE HYDROGELS; EMBRYO DEVELOPMENT; GROWTH;

D O I：

10.1387/ijdb.120175mz

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The ovarian follicle has a three-dimensional (3D) structure in which the oocyte is surrounded by tightly connected follicle cells that mediate the action of external signals and nourish the gamete during its maturation. Thus, the maintenance of follicle organization during the whole growth process is crucial for the correct acquisition of developmental competence. In recent years, much attention has been given to in vitro culture systems capable of maintaining follicle architecture. With the aim of providing a quick reference guide, in this review we will summarize the techniques developed for the 3D culture of mouse follicles.

引用

页码：931 / 937

页数：7

共 57 条

[11] Three dimensional culture of fresh and vitrified mouse pre-antral follicles in a hyaluronan-based hydrogel: a preliminary investigation of a novel biomaterial for in vitro follicle maturation [J].