Genotypic Diversity, Antibiotic Resistance and Bacteriocin Production of Enterococci Isolated from Rhizospheres

被引：10

作者：

Klibi, Naouel ^{[1
]}

Ben Slimen, Naouel ^{[1
]}

Fhoula, Imen ^{[1
]}

Lopez, Maria ^{[2
]}

Ben Slama, Karim ^{[1
]}

Daffonchio, Daniele ^{[3
]}

Boudabous, Abdellatif ^{[1
]}

Torres, Carmen ^{[2
]}

Ouzari, Hadda ^{[1
]}

机构：

[1] Fac Sci Tunis, Lab Microorganismes & Biomol Act, Dept Biol, Tunis 2092, Tunisia

[2] Univ La Rioja, Area Bioquim & Biol Mol, Logrono 26006, Spain

[3] Univ Milan, Dipartimento Sci & Tecnol Alimentari & Microbiol, I-20133 Milan, Italy

来源：

MICROBES AND ENVIRONMENTS | 2012年 / 27卷 / 04期

关键词：

Enterococci; antibiotic resistance; bacteriocins; MLST; PFGE; ANTIMICROBIAL RESISTANCE; VANCOMYCIN-RESISTANT; FECAL ENTEROCOCCI; VIRULENCE FACTORS; FAECIUM STRAINS; GENE-CLUSTER; VANA; SOIL; IDENTIFICATION; FAECALIS;

D O I：

10.1264/jsme2.ME12041

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

This study aimed to identify and to characterize rhizospheric-derived enterococci. The results showed the prevalence of Enterococcus faecium species (97%) vs. Enterococcus durans (3%). Susceptibility testing for antibiotics showed a low percentage of resistance to erythromycin (3.2%) and tetracycline (11.2%), and intermediate resistance to vancomycin (6.5%). Nevertheless, a high proportion of bacteriocin production was recorded. Furthermore, PCR detection of antibiotic resistance and bacteriocin production-encoding genes was investigated. Pulsed-field gel electrophoresis typing (PFGE) showed a great variability of enterococci in the rhizosphere. Moreover, mutilocus-sequence-typing analysis (MLST) revealed the identification of three new sequence types (STs), which were registered as ST613, ST614 and ST615.

引用

页码：533 / 537

页数：5

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