Conversion of 5-hydroxytryptophan into serotonin by tryptophan decarboxylase in plants, Escherichia coli, and yeast

被引：46

作者：

Park, Munyoung ^{[1
]}

Kang, Kiyoon ^{[1
]}

Park, Sangkyu ^{[1
]}

Back, Kyounowhan ^{[1
]}

机构：

[1] Chonnam Natl Univ, Biotechnol Res Inst, Agr Plant Stress Res Ctr, Dept Mol Biotechnol, Kwangju 500757, South Korea

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 2008年 / 72卷 / 09期

关键词：

tryptophan decarboxylase; 5-hydroxytryptophan decarboxylase; 5-hydroxytryptophan; serotonin;

D O I：

10.1271/bbb.80220

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The L-tryptophan decarboxylase (TDC) gene of rice was heterologously expressed in various organisms. Transgenic rice overexpressing TDC showed accumulation of serotonin upon 5-hydroxytryptophan treatment, which was consistent with the in vitro 5-hydroxytryptophan decarboxylase enzyme activity of purified recombinant rice TDC in a pyridoxal phosphate-dependent manner. Recombinant yeast harboring TDC produced serotonin at the expense of the endogenous 5hydroxytryptophan levels.

引用

页码：2456 / 2458

页数：3

共 6 条

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