Recognition of phospho sugars and nucleotides with an array of boronic acid appended bipyridinium salts

被引：38

作者：

Schiller, Alexander ^{[2
]}

Vilozny, Boaz ^{[1
]}

Wessling, Ritchie A. ^{[1
]}

Singaram, Bakthan ^{[1
]}

机构：

[1] Univ Calif Santa Cruz, Dept Chem & Biochem, Santa Cruz, CA 95064 USA

[2] Lab Protect & Physiol, Empa Swiss Fed Labs Mat Testing & Res, CH-9014 St Gallen, Switzerland

来源：

ANALYTICA CHIMICA ACTA | 2008年 / 627卷 / 02期

关键词：

Boronic acid; Nucleotide; Phospho sugar; Sensor array; Viologen; Fluorescence;

D O I：

10.1016/j.aca.2008.08.011

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The solution-phase sensor array of three cationic bis-boronic acid appended benzyl viologens (BBV) and the anionic fluorescent dye, 8-hydroxypyrene-1,3,6-trisulfonic acid trisodium. salt (HPTS), is able to discriminate among five phospho sugars, four nucleotides and three neutral saccharides in aqueous buffered solution at low mM concentrations. Linear discriminant analysis, principal component analysis, and hierachical cluster analysis studies showed the "discrimination limit" (lowest analyte concentration where the discrimination is still 100%) to be 4 mM. Calculated K-b and F-max/F-0 values from binding curves of the three BBVs with 1-12 were also used to perform multi-variate analyses with very good discrimination results. (C) 2008 Elsevier B.V. All rights reserved.

引用

页码：203 / 211

页数：9

共 42 条

[1] Cross-reactive chemical sensor arrays [J].

Albert, KJ ;

Lewis, NS ;

Schauer, CL ;

Sotzing, GA ;

Stitzel, SE ;

Vaid, TP ;

Walt, DR .

CHEMICAL REVIEWS, 2000, 100 (07) :2595-2626

[2] A calixpyridinium-pyranine complex as a selective anion sensing assembly via the indicator displacement strategy [J].

Atilgan, S ;

Akkaya, EU .

TETRAHEDRON LETTERS, 2004, 45 (50) :9269-9271

[3] Dynamic combinatorial libraries of dye complexes as sensors [J].

Buryak, A ;

Severin, K .

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2005, 44 (48) :7935-7938

[4] A chemosensor array for the colorimetric identification of 20 natural amino acids [J].

Buryak, A ;

Severin, K .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2005, 127 (11) :3700-3701

[5]

Buryak A, 2007, CHEM COMMUN, P2366, DOI 10.1039/b705250b

[6] A competition assay for determining glucose-6-phosphate concentration with a tris-boronic acid receptor [J].

Cabell, LA ;

Monahan, MK ;

Anslyn, EV .

TETRAHEDRON LETTERS, 1999, 40 (44) :7753-7756

[7] Boronic acid based peptidic receptors for pattern-based saccharide sensing in neutral aqueous media, an application in real-life samples [J].

Edwards, Nicola Y. ;

Sager, Thomas W. ;

McDevitt, John T. ;

Anslyn, Eric V. .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2007, 129 (44) :13575-13583

[8]

ELLIOTT WH, 2005, BIOCH MOL BIOL

[9] Anion binding involving π-acidic heteroaromatic rings [J].

Gamez, Patrick ;

Mooibroek, Tiddo J. ;

Teat, Simon J. ;

Reedijk, Jan .

ACCOUNTS OF CHEMICAL RESEARCH, 2007, 40 (06) :435-444

[10] Boronic acid-based bipyridinium salts as tunable receptors for monosaccharides and α-hydroxycarboxylates [J].

Gamsey, Soya ;

Miller, Aaron ;

Olmstead, Marilyn M. ;

Beavers, Christine M. ;

Hirayama, Lacie C. ;

Pradhan, Sulolit ;

Wessling, Ritchie A. ;

Singaram, Bakthan .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2007, 129 (05) :1278-1286

← 1 2 3 4 5 →