Application of a near-infrared laser tweezers Raman spectroscopy system for label-free analysis and differentiation of diabetic red blood cells

被引：24

作者：

Lin, Jinyong ^{[1
,2
]}

Shao, Lingdong ^{[1
,2
]}

Qiu, Sufang ^{[1
,2
]}

Huang, Xingwu ^{[1
,2
]}

Liu, Mengmeng ^{[3
,4
]}

Zheng, Zuci ^{[3
,4
]}

Lin, Duo ^{[3
,4
]}

Xu, Yongliang ^{[3
,4
]}

Li, Zhihua ^{[3
,4
]}

Lin, Yao ^{[5
]}

Chen, Rong ^{[3
,4
]}

Feng, Shangyuan ^{[3
,4
]}

机构：

[1] Fujian Canc Hosp, Dept Radiat Oncol, Fuzhou, Fujian, Peoples R China

[2] Fujian Med Univ Canc Hosp, Fuzhou, Fujian, Peoples R China

[3] Fujian Normal Univ, Key Lab OptoElect Sci & Technol Med, Minist Educ, Fuzhou 350007, Fujian, Peoples R China

[4] Fujian Normal Univ, Fujian Prov Key Lab Photon Technol, Fuzhou 350007, Fujian, Peoples R China

[5] Fujian Normal Univ, Prov Univ Key Lab Cellular Stress Response & Meta, Coll Life Sci, Fuzhou 350117, Fujian, Peoples R China

来源：

BIOMEDICAL OPTICS EXPRESS | 2018年 / 9卷 / 03期

基金：

中国国家自然科学基金;

关键词：

OPTICAL DIAGNOSIS; SENSOR;

D O I：

10.1364/BOE.9.000984

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A home-made near-infrared laser tweezers Raman spectroscopy (LTRS) system was applied to detect hemoglobin variation in red blood cells (RBCs) from diabetes without exogenous labeling. Results showed significant spectral differences existed between the diabetic and normal RBCs, including the peaks dominated by protein components (e.g. 1003 cm(-1)) and heme groups (e.g. 753 cm(-1)) in RBCs, and accurate classification results for diabetes detection were obtained by linear discriminant analysis with 100% sensitivity (i.e. no false negatives in the study). This work indicated the great promise of LTRS as a label-free RBC analytical tool for improving the accurate detection of type II diabetes. (C) 2018 Optical Society of America under the terms of the OSA Open Access Publishing Agreement

引用

页码：984 / 993

页数：10