The animal experimental is a fundamental equipment for biomechanics experimental, which attached to electric shock instrument, oxygen analyser or high speed recording system. The animal experimental has an advantage that it offers a single environment excluded the interfere from natural regimes. In this paper, a small animal observation experimental-bed was developed based on C++. The interface of control program was developed using Visual Basic 6.0 and the COM port communication between SCM LM3S8962 and PC was developed based on C++ Builder 6.0. The velocities of belt of the small animal experimental can run according to line, sinusoidal curves, triangular wave curves and square wave curves. Moreover, the control program provides a combined velocity based on above curves for satisfy the sophisticated experiment of locomotory small animals.