Reusable gold nanoparticle enhanced QCM immunosensor for detecting C-reactive protein

被引：30

作者：

Ding, Pengfei ^{[1
]}

Liu, Ruxiu ^{[1
]}

Liu, Shihui ^{[1
]}

Mao, Xueyin ^{[1
]}

Hu, Ruifen ^{[1
]}

Li, Guang ^{[1
]}

机构：

[1] Zhejiang Univ, Inst Cyber Syst & Control, Natl Lab Ind Control Technol, Hangzhou 310027, Zhejiang, Peoples R China

来源：

SENSORS AND ACTUATORS B-CHEMICAL | 2013年 / 188卷

关键词：

QCM; CRP; AuNP; Sensitivity enhancement; QUARTZ-CRYSTAL MICROBALANCE; CRP; IMMUNOASSAY; MARKERS; ASSAY;

D O I：

10.1016/j.snb.2013.07.099

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

This report describes a novel method to realize high sensitive C-reactive protein (hs-CRP) by using a quartz crystal microbalance (QCM) immunosensor based on 20 nm gold nanoparticles conjugated anti-CRP antibodies. A sandwich-type assay was established: CRP was firstly recognized by the anti-CRP antibody which was immobilized on the gold electrode of the QCM and the sensitivity was enhanced further by binding AuNP conjugated anti-CRP antibody secondly. The response of the QCM is observed to be linearly relative to the CRP concentration in the range of 20 ng/mL to 30 mu g/mL and the detection limit is enhanced to a great extend by the amplification procedure. The sensor was regenerated online by the solution of 3 mol/L Urea while preserving the activity of the immunosensor. (C) 2013 Elsevier B. V. All rights reserved.

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收藏

页码：1277 / 1283

页数：7

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