Thermosensitive bFGF-Modified Hydrogel with Dental Pulp Stem Cells on Neuroinflammation of Spinal Cord Injury

被引：63

作者：

Albashari, Abdullkhaleg ^{[2
]}

He, Yan ^{[1
,4
]}

Zhang, Yanni ^{[2
]}

Ali, Jihea ^{[5
]}

Lin, Feiou ^{[2
]}

Zheng, Zengming ^{[6
,7
]}

Zhang, Keke ^{[2
]}

Cao, Yanfan ^{[2
]}

Xu, Chun ^{[1
]}

Luo, Lihua ^{[2
]}

Wang, Jianming ^{[4
]}

Ye, Qingsong ^{[1
,2
,3
]}

机构：

[1] Univ Queensland, Sch Dent, Brisbane, Qld 4006, Australia

[2] Wenzhou Med Univ, Sch & Hosp Stomatol, Wenzhou 325035, Zhejiang, Peoples R China

[3] Wuhan Univ, Renmin Hosp, Ctr Regenerat Med, Wuhan 430060, Hubei, Peoples R China

[4] Wuhan Univ Sci & Technol, Tianyou Hosp, Lab Regenerat Med, Wuhan 430064, Hubei, Peoples R China

[5] Wenzhou Univ, Coll Life & Environm Sci, Wenzhou 325035, Zhejiang, Peoples R China

[6] Wenzhou Med Univ, Affiliated Hosp 2, Wenzhou 325000, Zhejiang, Peoples R China

[7] Wenzhou Med Univ, Yuying Childrens Hosp, Wenzhou 325000, Zhejiang, Peoples R China

来源：

ACS OMEGA | 2020年 / 5卷 / 26期

基金：

中国国家自然科学基金;

关键词：

MESENCHYMAL STEM; MOUSE MODEL; TRANSPLANTATION; RECOVERY; ACID; ACTIVATION; MICROGLIA; HEPARIN; REPAIR;

D O I：

10.1021/acsomega.0c01379

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Acute spinal cord injury (SCI) induces severe neuroinflammation, which increases intermediary filaments and neurodegeneration. Previous studies have shown that a basic fibroblast growth factor (bFGF) and dental pulp stem cells (DPSCs) contribute to a protective effect on injured neuronal cells, but the mechanism of SCI repair is still unclear. In this study, in situ heparin (HeP) hydrogel injection containing bFGF and DPSCs (HeP-bFGF-DPSCs), as well as in vitro studies of bFGF and DPSCs, proved an effective control over inflammation. The in vivo application of HeP-bFGF-DPSCs regulated inflammatory reactions and accelerated the nerve regeneration through micro- tubule stabilization and tissue vasculature. Our mechanistic investigation also showed that bFGF-DPSCs treatment inhibited microglia/macrophage proliferation and activation. Furthermore, HeP-bFGF-DPSCs prevented microglia/macrophage activation and reduced proinflammatory cytokine release. In this paper, we discovered that bFGF and DPSCs worked together to attenuate tissue inflammation of the injured spinal cord, resulting in a superior nerve repair. Our results indicated that a thermosensitive hydrogel delivering bFGF and DPSCs could serve as a promising treatment option for spinal cord injuries.

引用

页码：16064 / 16075

页数：12

共 50 条

[1] Inflammogenesis of Secondary Spinal Cord Injury [J].