Standardization and International Multicenter Validation of a PulseNet Pulsed-Field Gel Electrophoresis Protocol for Subtyping Shigella flexneri Isolates

被引：20

作者：

Pichel, Mariana ^{[1
]}

Brengi, Silvina P. ^{[1
]}

Cooper, Kara L. F. ^{[2
]}

Ribot, Efrain M. ^{[2
]}

Al-Busaidy, Suleiman ^{[3
]}

Araya, Pamela ^{[4
]}

Fernandez, Jorge ^{[4
]}

Vaz, Tania Ibelli ^{[5
]}

Kam, Kai Man ^{[6
]}

Morcos, Myriam ^{[7
]}

Nielsen, Eva M. ^{[8
]}

Nadon, Celine ^{[9
]}

Pimentel, Guillermo ^{[7
]}

Perez-Gutierrez, Enrique ^{[10
]}

Gerner-Smidt, Peter ^{[2
]}

Binsztein, Norma ^{[1
]}

机构：

[1] ANLIS Carlos G Malbran, Inst Nacl Enfermedades Infecciosas, Buenos Aires, DF, Argentina

[2] Ctr Dis Control & Prevent, Atlanta, GA USA

[3] Cent Publ Hlth Lab, Muscat, Oman

[4] Inst Salud Publ Chile, Santiago, Chile

[5] Adolfo Lutz Inst, Sao Paulo, Brazil

[6] Publ Hlth Lab Ctr, Kowloon, Hong Kong, Peoples R China

[7] US Naval Med Res Unit 3 NAMRU 3, Global Dis Detect GDD Reg Ctr, Cairo, Egypt

[8] Statens Serum Inst, DK-2300 Copenhagen, Denmark

[9] Natl Microbiol Lab, Winnipeg, MB, Canada

[10] PAHO WHO, VPH PANAFTOSA, Panama City, Panama

来源：

FOODBORNE PATHOGENS AND DISEASE | 2012年 / 9卷 / 05期

关键词：

OUTBREAK; SONNEI; INFECTION;

D O I：

10.1089/fpd.2011.1067

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

Shigella flexneri is one of the agents most frequently linked to diarrheal illness in developing countries and often causes outbreaks in settings with poor hygiene or sanitary conditions. Travel is one of the means by which S. flexneri can be imported into developed countries, where this pathogen is not commonly seen. A robust and discriminatory subtyping method is needed for the surveillance of S. flexneri locally and regionally, and to aid in the detection and investigation of outbreaks. The PulseNet International network utilizes standardized pulsed-field gel electrophoresis (PFGE) protocols to carry out laboratory-based surveillance of foodborne pathogens in combination with epidemiologic data. A multicenter validation was carried out in nine PulseNet laboratories located in North and South America, Europe, and Asia, and it demonstrated that a new protocol is highly robust and reproducible for subtyping of S. flexneri. This protocol, already approved for PulseNet laboratories, applies NotI and XbaI as primary and secondary restriction enzymes, respectively, under electrophoresis conditions of initial switch time of 5 s to final switch time of 35 s, at 6 volts/cm.

引用

页码：418 / 424

页数：7