Molecular identification of a phytoplasma associated with Sophora Root yellows

被引：4

作者：

Chen, X. F. ^{[1
,2
]}

Liang, Y. C. ^{[1
]}

Chen, N. ^{[1
]}

Su, W. M. ^{[1
]}

Xiao, H. ^{[1
]}

Wang, X. ^{[1
]}

Zhu, X. P. ^{[1
]}

机构：

[1] Shandong Agr Univ, Coll Plant Protect, Dept Plant Pathol, Tai An 271018, Shandong, Peoples R China

[2] Tarim Univ, Coll Plant Sci, Alar, Peoples R China

来源：

FOREST PATHOLOGY | 2013年 / 43卷 / 05期

基金：

中国国家自然科学基金;

关键词：

16S RIBOSOMAL-RNA; ASTER YELLOWS; FINER DIFFERENTIATION; CLASSIFICATION; DELINEATION; PHYLOGENY; SEQUENCES; DISEASE;

D O I：

10.1111/efp.12048

中图分类号：

S7 [林业];

学科分类号：

0829 ; 0907 ;

摘要：

A phytoplasma infecting Sophora Root (Sophora alopecuroides) was detected and identified in Alar, Xinjiang Uygur Autonomous Region of China. Typical phytoplasma bodies were observed in sieve tubes of the diseased plants by transmission electron microscopy. A partial 16S rRNA gene and ribosomal protein (rp) genes containing rpl22 (rplV) and rps3 (rpsC) were amplified by direct and nested PCR. Based on the sequence similarity of the 16S rRNA and rp genes with accompanying phylogenetic analyses, the phytoplasma associated with Sophora Root yellows belongs to the 16SrI group (aster yellows group). Virtual RFLP analysis of these 16S rRNA and rp gene sequences showed distinct differences from those of reference phytoplasma strains representing previously described subgroups of the 16SrI group. Moreover, the similarity coefficient (0.92) of the RFLP profile of this phytoplasma was less than the threshold similarity coefficient (0.97) required for subgroup classification. Thus, the phytoplasma isolate of Sophora Root plants, designated as SoRY', represents a new subgroup. Furthermore, this is the first report of phytoplasma disease associated with Sophora Root plants.

引用

页码：415 / 421

页数：7

共 22 条

[1] Variability and functional role of chromosomal sequences in 16SrI-B subgroup phytoplasmas including aster yellows and related strains [J].