Association of Interferon-gamma Induced Protein 10 Promoter Polymorphisms with the Disease Progression of Hepatitis B Virus Infection in Chinese Han Population

被引：17
作者：
Xu, Zhihui [1 ]
Liu, Yan [1 ]
Liu, Liming [2 ]
Li, Xiaodong [1 ]
Bai, Siyu [1 ]
Rong, Yihui [3 ]
Wang, Haibin [2 ]
Mao, Yuanli [2 ]
Xin, Shaojie [3 ]
Xu, Dongping [1 ]
机构：
[1] Beijing 302 Hosp, Viral Hepatitis Res Lab, Beijing, Peoples R China
[2] Beijing 302 Hosp, Ctr Clin Lab, Beijing, Peoples R China
[3] Beijing 302 Hosp, Med Ctr Liver Failure, Beijing, Peoples R China
来源：
PLOS ONE | 2013年 / 8卷 / 09期
基金：
中国国家自然科学基金;
关键词：
REGULATORY T-CELLS; HCV-GENOTYPE; IP-10; EXPRESSION; CXCL10; LIVER; GENE;
D O I：
10.1371/journal.pone.0072799
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Background and Aims: Interferon-gamma induced protein 10 (IP-10) was suggested to be involved in liver injury in viral hepatitis. This study aimed to investigate the impact of the single nucleotide polymorphisms (SNP) G-201A (rs1439490) in IP-10 gene on disease progression of hepatitis B virus (HBV) infection. Methods: The -201 SNP in IP-10 promoter was genotyped from 577 patients with different illness categories and 275 health controls; In vitro IP-10 promoter activity was compared between haplotype GG and AA homozygotes using luciferase reporter system in HepG2 cells. In vivo expression of IP-10 was compared between patients with -201 AA genotype and GG genotype. Results: The detected frequency of G-201A SNP was 17.8%, 25.3%, 26.6%, and 13.8% for patients with acute hepatitis B (AHB), patients with mild chronic hepatitis B (CHB-M), patients with severe chronic hepatitis B (CHB-S), and health controls, respectively. In vitro IP-10 promoter-driven luciferase activity in pGL3-Enhancer-201A transfected HepG2 cells was 1.43-fold higher than that in pGL3-Enhancer-201G transfected HepG2 cells (P<0.01). In vivo IP-10 transcriptional expression of peripheral blood mononuclear cells was 1.38-fold higher in patients with -201 AA genotype than in patients with -201 GG genotype (P<0.01). Conclusion: G-201A in promoter region of IP-10 gene was associated with liver disease progression in patients with HBV infection through up-regulating IP-10 expression.
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