A novel zinc finger protein is associated with U7 snRNP an interacts with the stem-loop binding protein in the histone pre-mRNP to stimulate 3′-end processing

被引：74

作者：

Dominski, Z ^{[1
]}

Erkmann, JA ^{[1
]}

Yang, XC ^{[1
]}

Sànchez, R ^{[1
]}

Marzluff, WF ^{[1
]}

机构：

[1] Univ N Carolina, Dept Biochem & Biophys, Program Mol Biol & Biotechnol, Chapel Hill, NC 27599 USA

来源：

GENES & DEVELOPMENT | 2002年 / 16卷 / 01期

关键词：

histone mRNA; two-hybrid; U7; snRNP; zinc finger; RNA binding;

D O I：

10.1101/gad.932302

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

The stem-loop binding protein (SLBP) is the posttranscriptional regulator of histone mRNA in metazoan cells. SLBP binds histone pre-mRNAs and facilitates 3'-end processing by promoting stable association of U7 snRNP with the pre-mRNA. To identify other factors involved in histone pre-mRNA processing, we used a modified yeast two-hybrid assay in which SLBP and its RNA target were coexpressed as bait. A novel zinc finger protein, hZFP100, which interacts with the SLBP/RNA complex but not with free SLBP, was cloned. The interaction requires regions of SLBP that are important for histone pre-mRNA processing. Antibodies to hZFP100 precipitate U7 snRNA, and expression of hZFP100 in Xenopus oocytes stimulates processing of histone pre-mRNA, showing that hZFP100 is a component of the processing machinery.

引用

页码：58 / 71

页数：14

共 69 条

[1] RNA-BINDING PROPERTIES AND EVOLUTIONARY CONSERVATION OF THE XENOPUS MULTIFINGER PROTEIN XFIN [J].