Preparative Isolation and Purification of Three Sesquiterpenoid Lactones from Eupatorium lindleyanum DC. by High-Speed Counter-Current Chromatography

被引：10

作者：

Yan, Guilong ^{[1
,2
,3
]}

Ji, Lilian ^{[2
,3
]}

Luo, Yuming ^{[2
]}

Hu, Yonghong ^{[1
]}

机构：

[1] Nanjing Univ Technol, Coll Biotechnol & Pharmaceut Engn, Nanjing 210009, Jiangsu, Peoples R China

[2] Huaiyin Normal Univ, Jiangsu Key Lab Ecoagr Biotechnol Hongze Lake, Huaian 223300, Peoples R China

[3] Huaiyin Normal Univ, Jiangsu Key Lab Biomass Based Energy & Enzyme Tec, Huaian 223300, Peoples R China

来源：

MOLECULES | 2012年 / 17卷 / 08期

关键词：

Eupatorium lindleyanum DC; high-speed counter-current chromatography; sesquiterpenoid lactone; separation; EXTRACTS; PLANT;

D O I：

10.3390/molecules17089002

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A high-speed counter-current chromatography (HSCCC) method was established for the preparative separation of three sesquiterpenoid lactones from Eupatorium lindleyanum DC. The two-phase solvent system composed of n-hexane-ethyl acetate-methanol-water (1: 4: 2: 3, v/v/v/v) was selected. From 540 mg of the n-butanol fraction of Eupatorium lindleyanum DC., 10.8 mg of 3 beta-hydroxy-8 beta-[4'-hydroxytigloyloxy]-costunolide, 17.9 mg of eupalinolide A and 19.3 mg of eupalinolide B were obtained in a one-step HSCCC separation, with purities of 91.8%, 97.9% and 97.1%, respectively, as determined by HPLC. Their structures were further identified by ESI-MS and H-1-NMR.

引用

页码：9002 / 9009

页数：8

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