Generation of a whole-brain atlas for the cholinergic system and mesoscopic projectome analysis of basal forebrain cholinergic neurons

被引：224

作者：

Li, Xiangning ^{[1
,2
]}

Yu, Bin ^{[3
,4
]}

Sun, Qingtao ^{[1
]}

Zhang, Yalun ^{[1
]}

Ren, Miao ^{[1
]}

Zhang, Xiaoyan ^{[1
]}

Li, Anan ^{[1
,2
]}

Yuan, Jing ^{[1
]}

Madisen, Linda ^{[5
]}

Luo, Qingming ^{[1
,2
]}

Zeng, Hongkui ^{[5
]}

Gong, Hui ^{[1
,2
]}

Qiu, Zilong ^{[2
,3
]}

机构：

[1] Huazhong Univ Sci & Technol, Minist Educ, Key Lab Biomed Photon, Sch Engn Sci,Wuhan Natl Lab Optoelect,Britton Chan, Wuhan 430074, Hubei, Peoples R China

[2] Chinese Acad Sci, Ctr Excellence Brain Sci & Intelligence Technol, Shanghai 200031, Peoples R China

[3] Chinese Acad Sci, State Key Lab Neurosci, Inst Neurosci, Shanghai 200031, Peoples R China

[4] Univ Chinese Acad Sci, Beijing 100049, Peoples R China

[5] Allen Inst Brain Sci, Washington, DC 98103 USA

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 2018年 / 115卷 / 02期

基金：

中国国家自然科学基金;

关键词：

cholinergic neurons; tomography; whole-brain imaging basal forebrain; single neuron reconstruction; MOUSE-BRAIN; IMMUNOCYTOCHEMICAL LOCALIZATION; CORTEX; ACETYLTRANSFERASE; TOMOGRAPHY; COGNITION; SLEEP;

D O I：

10.1073/pnas.1703601115

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The cholinergic system in the brain plays crucial roles in regulating sensory and motor functions as well as cognitive behaviors by modulating neuronal activity. Understanding the organization of the cholinergic system requires a complete map of cholinergic neurons and their axon arborizations throughout the entire brain at the level of single neurons. Here, we report a comprehensive whole-brain atlas of the cholinergic system originating from various cortical and subcortical regions of the mouse brain. Using genetically labeled cholinergic neurons together with whole-brain reconstruction of optical images at 2-mu m resolution, we obtained quantification of the number and soma volume of cholinergic neurons in 22 brain areas. Furthermore, by reconstructing the complete axonal arbors of fluorescently labeled single neurons from a subregion of the basal forebrain at 1-pm resolution, we found that their projections to the forebrain and midbrain showed neuronal subgroups with distinct projection specificity and diverse arbor distribution within the same projection area. These results suggest the existence of distinct subtypes of cholinergic neurons that serve different regulatory functions in the brain and illustrate the usefulness of complete reconstruction of neuronal distribution and axon projections at the mesoscopic level.

引用

页码：415 / 420

页数：6

共 35 条

[1] DISTRIBUTION OF CHOLINERGIC NEURONS IN RAT-BRAIN - DEMONSTRATED BY THE IMMUNOCYTOCHEMICAL LOCALIZATION OF CHOLINE-ACETYLTRANSFERASE [J].