Real-Time Quantitative Monitoring of Specific Peptide Cleavage by a Proteinase for Cancer Diagnosis

被引：26

作者：

Lee, Gyudo ^{[1
,2
]}

Eom, Kilho ^{[1
,2
]}

Park, Joseph ^{[3
]}

Yang, Jaemoon ^{[1
,4
]}

Haam, Seungjoo ^{[3
]}

Huh, Yong-Min ^{[4
]}

Ryu, Joo Kyung ^{[5
]}

Kim, Nam Hee ^{[5
]}

Yook, Jong In ^{[5
]}

Lee, Sang Woo ^{[2
]}

Yoon, Dae Sung ^{[2
]}

Kwon, Taeyun ^{[1
,2
]}

机构：

[1] Inst Mol Sci, Seoul 120749, South Korea

[2] Yonsei Univ, Dept Biomed Engn, Wonju 220710, South Korea

[3] Yonsei Univ, Dept Chem & Biomol Engn, Seoul 120749, South Korea

[4] Yonsei Univ, Dept Radiol, Seoul 120752, South Korea

[5] Yonsei Univ, Dept Oral Pathol, Oral Canc Res Inst, Seoul 120752, South Korea

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2012年 / 51卷 / 24期

基金：

新加坡国家研究基金会;

关键词：

atomic force microscopy; biosensors; cancer; peptides; proteases; TYPE-1; MATRIX-METALLOPROTEINASE; MMP EXPRESSION; CELLS; BREAST;

D O I：

10.1002/anie.201108830

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Good vibrations: A bioassay using a resonant peptide-functionalized microcantilever enables the quantitative characterization of the proteolytic activity of membrane type-1 matrix metalloproteinase (MT1-MMP). In this assay, shifts in the frequency of the cantilever after specific proteolytic cleavage of the target peptides by MT1-MMP are measured (see picture). Copyright © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

引用

页码：5837 / 5841

页数：5

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