Isolation of circulating tumor cells

被引：44

作者：

Edd, Jon F. ^{[1
,2
,3
]}

Mishra, Avanish ^{[1
,2
,3
,5
]}

Smith, Kyle C. ^{[4
]}

Kapur, Ravi ^{[1
,2
,4
]}

Maheswaran, Shyamala ^{[3
,5
]}

Haber, Daniel A. ^{[3
,5
,6
]}

Toner, Mehmet ^{[1
,2
,5
,7
]}

机构：

[1] Massachusetts Gen Hosp, BioMEMS Resource Ctr, Ctr Engn Med & Surg Serv, Boston, MA 02114 USA

[2] Harvard Med Sch, Boston, MA 02114 USA

[3] Massachusetts Gen Hosp, Canc Ctr, Boston, MA 02114 USA

[4] BendBio Inc, Sharon, MA 02067 USA

[5] Harvard Med Sch, Boston, MA 02115 USA

[6] Howard Hughes Med Inst, Bethesda, MD 20815 USA

[7] Shriners Hosp Children, Boston, MA 02114 USA

来源：

ISCIENCE | 2022年 / 25卷 / 08期

关键词：

CANCER-PATIENTS; DIAGNOSTIC LEUKAPHERESIS; LIQUID BIOPSY; LABEL-FREE; BLOOD; SEPARATION; ENRICHMENT; CLUSTERS; PARTICLE; SYSTEM;

D O I：

10.1016/j.isci.2022.104696

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Circulating tumor cells (CTCs) enter the vasculature from solid tumors and disseminate widely to initiate metastases. Mining the metastatic-enriched molecular signatures of CTCs before, during, and after treatment holds unique potential in personalized oncology. Their extreme rarity, however, requires isolation from large blood volumes at high yield and purity, yet they overlap leukocytes in size and other biophysical properties. Additionally, many CTCs lack EpCAM that underlies much of affinity-based capture, complicating their separation from blood. Here, we provide a comprehensive introduction of CTC isolation technology, by analyzing key separation modes and integrated isolation strategies. Attention is focused on recent progress in microfluidics, where an accelerating evolution is occurring in high-throughput sorting of cells along multiple dimensions.

引用

页数：15

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